NK细胞对小鼠H2单倍体相合骨髓移植后GVHD及造血重建的影响
杀伤细胞,天然;,骨髓移植;,移植物抗宿主病;,造血重建,,杀伤细胞,天然;,骨髓移植;,移植物抗宿主病;,造血重建,【关键词】杀伤细胞,天然;骨髓移植;移植物抗宿主病;造血重建,0引言,1材料和方法,2结果,3讨论,【参考文献
Effect of NK cell on GVHD and hematopoietic reconstitution after H2 haploidentical bone marrow transplantation in miceZHANG Mei, WU Di, XU Hui, HE PengCheng, LI Jing, XI JieYing, CAI RuiBo, LIU YaLin
Department of Hematology, First Affiliated Hospital, Medical College, Xian Jiaotong University, Xian 710061, China
【Abstract】 AIM: To explore the effect of natural killer (NK) cell on graftversushost disease (GVHD) and hematopoietic reconstitution after H2 haploidentical bone marrow transplantation in mice. METHODS: Murine model of H2 haplotype mismatched bone marrow transplantation was established by using Balb/c (H2d) mouse as recipient, and Balb/c (H2d)×C57BL/6(H2b)(H2d/b) mouse as donor. Lethally irradiated Balb/c (H2d) mice were transplanted with Balb/c (H2d)×C57BL/6(H2b)(H2d/b) bone marrow plus with donor peripheral T cells and/or NK cells. GVHD and hematopoietic reconstitution were observed and evaluated by clinical manifestations, pathological changes and WBC counting. RESULTS: In the bone marrow plus with T cells group, GVHD was induced in 90% mice and the recovery of the WBC needed 4 weeks averagely; but in the group plus with low concentration of NK cells, GVHD was induced in 20% mice and the recovery of the WBC was 3 weeks averagely; and in the group transplanted with high concentration of NK cells, GVHD was induced only in 10% mice and the recovery of the WBC was 3 weeks averagely. Compared with the group transplanted only with T cells, the incidences of GVHD in the later two groups decreased obviously (P<0.01), and the recovery of the WBC was in a shorter time (P<0.01). CONCLUSION: In mouse H2 haploidentical bone marrow transplantation, alloreactive NK cells can reduce the incidence of GVHD and promote hematopoietic reconstitution. ......
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