【摘要】 目的 通过对中国华东地区分离出的2株基因3型戊型肝炎病毒株进行同源性分析，探讨其分子起源和传播问题。 方法 收集华东某地区13个养猪场的133份猪粪便标本，利用巢式RT-PCR技术扩增戊肝病毒基因组的开放读码区2(ORF2)和RNA依赖的RNA聚合酶区(RdRp，ORF1)，并进行基因同源性分析。 结果 戊肝病毒RNA检出率为43.61%(58/133)。其中，在同一个养猪场检出的2份阳性标本与23条戊肝病毒基因3型全长序列在ORF2和ORF1区的同源性分别为77.10%～92.64%和77.49%～91.14%，被划分为基因3型。另外12个养猪场的56份阳性标本均为基因4型。同时发现，某些日本分离的基因3型病毒株比其他基因3型株更接近本次分离的病毒株。 结论 这是在中国大陆地区首次发现基因3型戊肝病毒。同时证明在一个较为局限的群体中基因3型和4型病毒可以共存。

    【关键词】 肝炎病毒，戊型;基因型;序列同源性，核酸

    Identification of the genotypeⅢhepatitis E virus from swine herds in China

    LU Yi-han 1 ，ZHENG Ying-jie 1 ，ZHU Jian-fu 2 ，WANG Fa-di 2 ，XIA Ning-shao 3 ，JIANG Qing-wu 1 .

    1.Key Laboratory of Public Health Safety，Ministry of Education-Department of Epidemiology，School of Public Health，Fudan University，Shanghai 200032，China;2.Center for Disease Control and Prevention，De-qing County，Deqing 313200，China;3.Research Center on Medical Molecular Virology in Fujian，Xiamen University，Xiamen 361005，China

    【Abstract】 Objective To determine the phylogenetic reconstruction of two genotype-III HEV strains isolated in Eastern China and investigate the molecular ancestor and source for transmission.Methods A total of133swine fecal specimens was collected randomly from13local swine farms in Eastern China.The fragments in Open Reading Frame2(ORF2)and RNA-dependent RNA polymerase(RdRp)do-main in ORF1of hepatitis E virus genome were amplified with nested RT-PCR.Phylogenetic recon-struction of partial sequences was then performed based on them.Results HEV RNA was detected from43.61%(58/133)of fecal specimens.In one swine farm ......