I型胶原；氟斑牙；基因多态性_

COL1A2基因多态性与儿童氟斑牙关系

http://www.100md.com 《中国公共卫生》 2007年第4期

I型胶原；氟斑牙；基因多态性,,I型胶原；氟斑牙；基因多态性,1对象与方法,2结果,3讨论,参考文献

     摘要：目的调查I型胶原α2链(COL1A2)PvuⅡ基因型在饮水氟、碘浓度不同地区儿童中的分布，探讨COL1A2 PvuⅡ基因多态性与儿童氟斑牙的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测氟碘正常区、单纯高氟区和高氟高碘区8～12岁儿童的COL1A2 PvuⅡ基因型。Dean氏法检查儿童氟斑牙患病情况。结果 3区儿童COL1A2 PvuⅡ基因型在氟碘正常地区分布频率分别为PP233%，Pp367%，pp400%；单纯高氟区分布频率分别为PP98%，Pp451%，pp451%，高氟高碘区分布频率分别为PP137%，Pp384%，pp479%，3区分布差异无统计学意义(χ2＝5191，P>005)。儿童氟斑牙患病率分别为0，527%，507%，差异有统计学意义(χ2＝49081，P<005)；单纯高氟区和高氟高碘区氟斑牙患病率差异无统计学意义(χ2＝0060，P>005)，但高氟高碘区的氟斑牙指数低于单纯高氟区。结论 COL1A2 PvuⅡ基因多态性与所调查儿童氟斑牙无明显关系。

    关键词： I型胶原；氟斑牙；基因多态性

    Relationship of COL1A2 polymorphism to children's dental fluorosis

    HUANG Hui,JIA Xinmei,LI Shihong,et al.

    Department of Environmental Health,College of Public Health ,Zhengzhou University (Zhengzhou 450001,China)

    Abstract： Objective To explore the distribution of collagen type 1 alpha2 (COL1A2)PvuⅡ genotype in children who lived in the high fluoride,high iodine areas,and evaluate the relationship of COL1A2 PvuⅡgene polymorphism to children's dental fluorosis.Methods Polymerase chain reaction-restriction fragment length polymorphism technique(PCR-RFLP) was used to determine COL1A2 PvuⅡ genotype of the different children lived in the normal fluoride and iodine area ,the high fluoride area,the high fluoride and high iodine area.The incidence of dental fluorosis was examined by using Dean's method.Results The distribution frequency of COL1A2 PvuⅡ genotype was PP23.3% ......

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