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天山岩黄芪根中多糖的提取及含量测定
http://www.100md.com 《时珍国医国药》 2007年第3期
天山岩黄芪根;,多糖含量;,分光光度法,,天山岩黄芪根;,多糖含量;,分光光度法,1材料与试剂,2方法与结果,3讨论,参考文献:
     摘要:目的研究天山岩黄芪根中多糖的含量。方法采用分光光度法测定天山岩黄芪根中多糖的含量,以葡萄糖为对照品,以苯酚-浓硫酸为显色剂,在486 nm处测定样品溶液的吸光度。结果标准曲线为A=51.654C + 0.037 2,r=0.999 8,RSD为 1.28%,1.50%。结论该方法操作简单,灵敏度高。

    关键词:天山岩黄芪根; 多糖含量; 分光光度法

    Determination of the Polysaccharide in the Roots of Hedysarum semenovii Regelet Herd

    Hailiqian·Taoerdahong, SUN Lian, ABUDOUXUKUER Tuerxun, HE Yan

    (Xinjiang Medical Univesrsity,Urumqi, Xinjiang 830054,China)

    Abstract:ObjectiveTo study the quantity of Polysaccharide in the roots of Hedysarum semenovii Regelet Herd. MethodsUses method in spectrophotometric to determine the Polysaccharide in the roots of Hedysarum semenovii Regelet Herd.Take the glucose as the comparison, take the phenol - strong sulfuric acid as the developer, λ= 486nm determination sample solution attracts the luminosity. ResultsThe standard curve was A=51.654C+0.037 2,r=0.999 8, RSD was 1.28% and1.5%(n=5). ConclusionThis method is simple,sensitive and accurate.

    Key words:Hedysarum semenovii Regelet Herd; Polysaccharide; SX-spectrophotometry

    豆科岩黄芪属植物 ......

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