不同产地山药rDNA ITS区序列的比较
山药；,ITS区序列；,聚合酶链反应,,山药；,ITS区序列；,聚合酶链反应,不同产地山药rDNAITS区序列的比较,1材料与方法,2结果,3讨论,参考文献：
摘要：目的分析怀山药与其它产地山药的rDNA ITS区序列的差异，为山药的基源鉴定和品质评价提供分子依据。方法采用PCR扩增纯化后直接测序的方法测定不同产地山药的rDNA 基因ITS核苷酸序列并作序列同源性分析。结果8种产地山药的ITS1片段长度均为165bp，ITS2 片段长度均为158~160bp, 5.8S 片段长度为均为159bp。8种不同产地的山药ITS1和ITS2区分别有6个和9个碱基变异。结论利用ITS 区序列的差异鉴别不同产区的山药提供了依据。关键词：山药； ITS区序列； 聚合酶链反应
Comparison of Ribosomal DNA ITS Sequence of Rhizoma Dioscoreae in Different Geographical Regions
WANG Dong,BAI Yan,CHEN Zhihong
(Henan College of Traditional Chinese Medicine, Zhengzhou, Henan 450008,China)
Abstract：ObjectiveTo distinguish Dioscoreaeu opposita Thunb. from different geographical regions by comparing Ribosomal DNA ITS sequence. MethodsDirect PCR sequencing was used to detect the homology of rDNA ITS sequence.ResultsEight Dioscoreae opposita Thunb. were obtained from different geographical regions by PCR technology. Sequence analysis showed that ITS1 was 165bp, ITS2 was 158~160bp and 5.8S was 159bp. There were 6 various sites in ITS1 and 9 various sites in ITS2 .ConclusionIt provides the basis for identifying Dioscoreae opposita Thunb. from different geographical regions according to the variation of the ITS sequences. ......
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