银黄清口服液；_总绿原酸；_二阶导数光谱法_

二阶导数光谱法测定银黄清口服液中总绿原酸含量

http://www.100md.com 《时珍国医国药》 2007年第2期

银黄清口服液；,总绿原酸；,二阶导数光谱法,,银黄清口服液；,总绿原酸；,二阶导数光谱法,1仪器与试药,2方法与结果,3讨论,参考文献：

     摘要：目的建立二阶导数光谱法测定银黄清口服液中总绿原酸含量的方法。方法用二阶导数光谱法直接测定银黄清口服液中总绿原酸含量，于357.9 nm波长处测定振幅D值。结果绿原酸在4.67~23.35 μg·ml1范围呈良好线性关系；其回归方程D=0.749 5×104C-0.1×10-4(r=0.997 2)；平均回收率97.88%，RSD=2.43%(n=5)。结论该方法操作简单、准确，可用于测定银黄清口服液中总绿原酸含量。

    关键词：银黄清口服液；总绿原酸；二阶导数光谱法

    Determination of Total Chlorogenic Acid in Yinhuangqing Oral Liquid by Second Order Derivative Spectrophotometry

    ZHOU Bolin, FENG Guodong

    (Beihai People's Hospital, Beihai, Guangxi 536000, China)

    Abstract：ObjectiveTo determine the content of total chlorogenic acid in Yinhuangqing oral liquid.MethodsSecond order derivative spectrophotometry was used.The peak amplitude was determined when the wavelength was at 357.9 nm.ResultsThe chlorogenic acid showed a good linear relationship within a range of 4.67~23.35 μg·ml1,the regression equation was D=0.749 5×104C-0.1×104(r=0.9972);the average recovery was 102.23% and RSD was 2.7%(n=5).ConclusionThe method is simple and accurate, and can be used for determination of total chlorogenic acid in Yinhuangqing Oral Liquid. ......

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