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杜仲叶中绿原酸的提取纯化研究
http://www.100md.com 《时珍国医国药》2007年第2期
绿原酸;,提取;,纯化;,杜仲叶,,绿原酸;,提取;,纯化;,杜仲叶,1材料与方法,2结果,3结论,参考文献:
     摘要:目的研究杜仲叶中绿原酸的提取纯化方法。方法采用水提、絮凝脱色、树脂吸附、重结晶工艺提取纯化。结果杜仲叶的水浸提液浓缩后用1%壳聚糖絮凝去杂、活性碳脱色得到进样液,进样液用NKA-9树脂吸附,50%乙醇解吸,洗脱液浓缩后的粗品经甲醇重结晶得到纯度≥97%的绿原酸,提取率≥65%。结论 优化完善了杜仲叶中绿原酸的提取纯化方法,为杜仲资源的充分综合利用和产业化开发提供了参考。

    关键词:绿原酸; 提取; 纯化; 杜仲叶

    Study on the Extraction and Purification of Chlorogenic Acid in Eucommia ulmoides Oliv. Leaves

    YUAN Hua, DENG Liang, YANG Xiaojun, YAN Zhiguo, WU Yuanxin

    (School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China)

    Abstract:Objective To study the optimum extraction and purification process for chlorogenic acid in Eucommia ulmoides Oliv. Leaves. MethodsThe process was carried out by water extraction, 1% chitosan flocculation, actived char decolorization, NKA9 resin adsorption, 50% ethanol desorption and methanol recrystallization. ResultsThe extracting ratio of chlorogenic acid was 68% with purity of 97%.ConclusionAn optimum and improved technology for the extraction and purification of chlorogenic acid in Eucommia ulmoides Oliv. Leaves was investigated. It is helpful to industrial production of chlorogenic acid. ......

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