毛老虎；_分光光度法；_香草醛____

分光光度法测定毛老虎茎中三萜酸的总含量

http://www.100md.com 《时珍国医国药》 2007年第2期

毛老虎；,分光光度法；,香草醛,,毛老虎；,分光光度法；,香草醛,1器材,2方法,3结果,4讨论,参考文献：

     摘要：目的建立香草醛-冰醋酸体系分光光度法测定毛老虎中总三萜酸的方法。方法利用在高氯酸的条件下香草醛-冰醋酸与三萜酸的反应测定总三萜酸。结果总三萜酸在2.53～13.14 μg/ml呈良好的线性关系，测定样品回收率为99.07%。结论该法简便、快速、灵敏。

    关键词：毛老虎；分光光度法；香草醛

    Determination of Total Content of Triterpenoids in Stems of Hyptis suaveolens (L.) Piot by Spectrophotometry

    HUANG Xiuxiang, YUAN Xia, LIU Cuiwu*, CHEN Lifen

    (School of Chemistry and Chemical Engineering, Guangxi University,Nanning 530004,China;Department of Chemistry and Life Sciences, Hechi College, Yizhou 546300,China)

    Abstract：ObjectiveTo develop the determination method of total triterpenoids in vanillinglacial acetic acid system by spectrophotometry.MethodsVanillin was used as colour developing reagent in the presence of perchloric acid.ResultsThe calibration curve of total triterpenoids had good linear relationship in the range of 2.53～13.14 μg/ml. The average recovery was 99.07%.ConclusionThe method is simple ,easy and reproducible .

    Key words：Hyptis suaveolens (L.)Piot; Spectrophotometry; Vanillin

    毛老虎Hyptis suaveolens (L.)Piot为唇形科山香属植物 ......

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