针刺；_脑缺血_再灌注；_P选择素；_E选择素_

电针对大鼠脑缺血再灌注模型P选择素和 E选择素表达影响的实验研究

http://www.100md.com 《时珍国医国药》 2007年第1期

针刺；,脑缺血,再灌注；,P选择素；,E选择素,,针刺；,脑缺血,再灌注；,P选择素；,E选择素,电针对大鼠脑缺血再灌注模型P选择素和E选择素表达影响的实验研究,1材料与方法,2结果,3讨论,参考文献：

     摘要：目的探讨针刺抗脑缺血再灌注炎性损伤的机理。方法采用线栓法制作大鼠大脑中动脉缺血模型(MCAO)，采用免疫组织化学技术测定P-选择素和E-选择素表达的动态变化。结果在灌注3 h各实验组P选择素和E选择素均开始表达，二者均于再灌注24 h均达到高峰(组内比较P<0.01)，持续至再灌注48 h，针刺可显著降低P-选择素和E-选择素的表达(与模型组和非穴组比较，P<0.01 )。结论P选择素和E选择素的表达上调与再灌注后的炎症反应有关，早期针刺治疗可能通过下调脑缺血区黏附分子P选择素和E选择素的表达而防治脑缺血再灌注炎性损伤。

    关键词：针刺；脑缺血/再灌注； P选择素； E选择素

    Effect of Galvano-acupuncture on the Expression of P-selectin and E-selectin of Cerebral Ischenic Region in Rats

    LIU Yuzhen, HAN Jingxian, JIANG Geli, DING Xiaorong, LU Mingxia

    (Restored Center of Tianjin Sanatorium, Beijing Military District, Tianjin 300381, China; Research Room for Aged Diseases, First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China)

    Abstract：ObjectiveTo study mechanisms of early treatment of cerebral ischemia-reperfusion(I/R) by galvano-acupuncture in resisting inflammation injury in rats.MethodsCerebral I/R model was established by occlusion of the middle cerebral artery for 1h and then removal of the occlusion for 3 h ,12 h,24 h, and 48 h respectively .The expression of P-selectin and Eselectin was displayed by using imunohistochemical ABC in ischemic cerebral cortex and corpus striatum in rats.ResultsThe expression of P-selectin and Eselectin was detected at capillary walls in postischemic cerbral cortex at 1 h ischemia-3 h reperfusion and peaked at 1h ischemia-24 h reperfusion and increased continuously during 48 h reperfusion.The expression of both Pselectin and Eselectin in ischemic cerebral cortex were downregulated considerably by galvano-acupuncture treatment (P<0.01) .ConclusionThe protective effect of acupuncture on cerebral neurons under acute infarction is possible through its downregulative action on the expression of Pselectin and Eselectin,which causes neutrophil infiltration that induced by adhesive molecules between endothelium cell and neutrophil. ......

百拇医药网 http://www.100md.com/html/DirDu/2007/04/28/43/02/76.htm

您现在查看是摘要页，全文长 9413 字符。