佛手；_香橼；_rDNA_ITS序列____

广佛手rDNA ITS序列测定及特点的初步分析

http://www.100md.com 《时珍国医国药》 2007年第1期

佛手；,香橼；,rDNA,ITS序列,,佛手；,香橼；,rDNA,ITS序列,广佛手rDNAITS序列测定及特点的初步分析,1材料和方法,2结果,3讨论,参考文献：

     摘要：目的分析广佛手及其近缘种香橼的rDNA ITS序列及其规律。方法采用PCR直接测序技术测定广佛手和香橼rDNA ITS序列并作序列变异分析。结果4个植物样品rDNA ITS序列长度为644~672 bp。其中5.8S rDNA 长度为165 bp，编码区较保守。广佛手和香橼的ITS1和ITS2序列平均遗传距离分别为0.009 5和0.014 2。结论rDNA ITS序列可为鉴定佛手提供分子参照系统。

    关键词：佛手；香橼； rDNA ITS序列

    Preliminary Study on rDNA ITS Sequencing and Characteristics of Citrus medica L.var.sarcodactylis(Noot.)Swingle

    GAO Xiaoxia, CHEN Xiaoying, LUO Yuansheng

    (Guangdong University of Pharmacy, Guangzhou510006,China;Guangdong Guang-Hong Medicine ＆ Drug Company Limited,Guangzhou510089,China)

    Abstract:ObjectiveThe ribosomal DNA internao transcribed spacers(ITS)and 5.8S rDNA ITS sequence of Citrus medica L.var.sarcodactylis(Nootc).Swingle and C.medicaL. were sequenced.MethodsThe rDNA ITS gene fragment was amplified by PCR with universal primers of rDNA ITS and sequenced.ResultsThe DNA sequence of ITS ranged from 644 bp to 672 bp and the 5.8S gene of all of the samples was 165 bp in size.Distance values of ITS1 and ITS2 sequence,estimated according to Kimura two-parameter models between Citrus medicaL.var.sarcodactylis(Noot.)Swingle and its adulterant and C.medica L.,were 0.0095 and 0.0142,respectively.ConclusionrDNA ITS sequence may be the evidence for the molecular authentication of Citrus medica L.var.sarcodactylis(Noot.)Swingle. ......

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