离子对反相高效液相色谱；_莲心碱；

高效液相色谱法同时测定莲子心中四种异喹啉生物碱的含量

http://www.100md.com 《时珍国医国药》 2005年第12期

离子对反相高效液相色谱；,莲心碱；,异莲心碱；,甲基莲心碱；,莲心季铵碱；,莲子心,,离子对反相高效液相色谱；,莲心碱；,异莲心碱；,甲基莲心碱；,莲心季铵碱；,莲子心,1实验材料,2方法与结果,3结果与讨论,参考文献：

     摘要：目的：建立同时测定莲子心中四种异喹啉生物碱莲心季铵碱、莲心碱、异莲心碱、甲基莲心碱含量的离子对反相高效液相色谱(RPHPLC)法。方法：用十八烷基硅烷键合硅胶为填充剂，色谱柱：HibarTR，5 μm，250 mm×4.6 mm；流动相：乙腈水冰醋酸(55∶45∶1，每1 000 ml中加入4 g十二烷基磺酸钠)；流速：1.2 ml/min；检测波长：282 nm；柱温：30℃。结果：莲心季铵碱在0.94～2.82 μg，莲心碱在1.14～3.42 μg，异莲心碱在0.7～2.1 μg，甲基莲心碱在0.96～2.88 μg范围内均呈良好线性关系；并均具有较好的加样回收率(>95%)。结论：该分析方法快速、准确、灵敏度高，重复性好，可用于同时对药材莲子心中四种异喹啉生物碱的含量测定。

    关键词：离子对反相高效液相色谱；莲心碱；异莲心碱；甲基莲心碱；莲心季铵碱；莲子心

    Determination of Four Isoquinoline Alkaloids in Plumula Nelumbini by HPLC Simultaneously

    PAN Yang， YANG Guangming， CAI Baochang

    (Pharmaceutical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China)

    Abstract:Objective:An ionpair RPHPLC method was established to determine the amounts of lotusine, liensinine, isoliensinine and neferine in Plumula Nelumbini(PNN)simultaneously.Methods:The column is packed with Hibar RT C18(5 μm, 250 mm×4.60 mm);The mobile phase consists of acetonitrile/H2O/glacial acetic acid(55∶45∶1)，with 4 g sodium lauryl sulfonate in 1 liter, at the flow rate of 1.2 ml/min and the detection wavelength is 282 nm while the column temperature is 30℃.Results:Lotusine, liensinine, isoliensinine and neferine were proved to be linear within the range of 0.94～2.82 μg, 1.14～3.42 μg,0.7～2.1 μg and 0.96～2.88 μg respectively,with the recovery of the four alkaloids all being above 95%.Conclusion:This method is feasible to determine these four isoquinoline alkaloids simultaneously in PNN with the advantages of quickness, accuracy, high sensitivity and good repeatability. ......

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