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高效液相色谱法测定参芪健胃颗粒中芍药苷的含量
http://www.100md.com 《时珍国医国药》 2007年第3期
参芪健胃颗粒;,芍药苷;,高效液相色谱法,,参芪健胃颗粒;,芍药苷;,高效液相色谱法,1仪器与试剂,2方法与结果,3讨论,参考文献:
     摘要:目的测定参芪健胃颗粒中芍药苷的含量。方法采用高效液相色谱法(HPLC)测定含量,色谱柱:Kromasil 1005 C18柱(4.6 mm×250 mm)。流动相:甲醇水(38∶62),检测波长:230 nm,流速:0.7 ml/min,柱温:30℃。结果该条件下芍药苷与其它组分分离良好,芍药苷在0~50 μg/ml呈良好的线性关系,r=0.999 2,平均回收率为97.90%,RSD=1.186%。3批供试品含芍药苷分别为0.024 2%,0.027 4%,0.021 7%,平均为0.024 4%。结论HPLC法可用于参芪健胃颗粒的质量控制。

    关键词:参芪健胃颗粒; 芍药苷; 高效液相色谱法

    Determination of Paeoniflorin in Shenqijianwei Granule by HPLC

    YU Shuzhen,ZHANG Hongmei, LU Chunyan

    (Jiangsu Zhongxing Pharmaceutical Limited Company,Zhenjiang 212009,China)

    Abstract:ObjectiveTo establish the determination method of Shenqijianwei Granule. MethodsHPLC was adopted with Kromasil 100-5 C18(4.6 mm×250 mm).The mobile phase corsrsted of acetonitrile:Methanolwater(38∶62). The detection wavelength was at 230 nm. The flow rate was 0.7 ml/min, and the column temperature was at 30℃.ResultsThe linearity of this method was well with a good seperation . The calibration curve was linear (r=0.999)in the range of 0~50μg/ml for Paeoniflorin. The average recovery was 97.90% ......

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