反相高效液相色谱法测定关节克痹丸中大黄素的含量
反相高效液相色谱法;,关节克痹丸;,大黄素,,反相高效液相色谱法;,关节克痹丸;,大黄素,1仪器与材料,2方法与结果,3小结,参考文献:
摘要:目的建立反相高效液相色谱(RP-HPLC)法测定关节克痹丸中大黄素的含量。方法采用Zobax C18色谱柱(4.6 mm×150 mm,5 μm),以甲醇0.1%磷酸溶液(70∶30)为流动相,流速1.0 ml/min,检测波长282 nm,柱温40℃。结果大黄素在0.01~0.2 mg/ml范围内线性关系良好(r=0.999 9),平均回收率为97.56%,RSD为1.51%。结论 该法操作简便,结果准确、可靠,可作为关节克痹丸的质量控制标准之一。关键词:反相高效液相色谱法; 关节克痹丸; 大黄素
Determination of Emodin in Guanjiekebi Pill by RP-HPLC
GU Zhengrong,CHENG Chen
(Changzhou Institute for Drug Control,Changzhou 213002,China)
Abstract:ObjectiveAn RP-HPLC method was established for the determination of emodin content in Guanjiekebi pill.MethodsZobax C18(4.6 mm×150 mm,5 μm) was used with a mobile phase consisted of methanol-0.1% phosphoric acid(70∶30).The detection wavelength was 282 nm,the flow rate was 1.0 ml/min,and the temperature of the column was 40℃.ResultsThe linear range of emodin was 0.01~0.2 mg/ml(r=0.999 9),while the average recovery was 97.56%(RSD=1.51%).ConclusionThe method is simple,sensitive and accurate.It can be applied in quality control of Guanjiekebi pill.
Key words:RP-HPLC; Guanjiekebi pill; Emodin
关节克痹丸由川乌(制)、草乌(制)、虎杖、独活、秦艽、麻黄等15味中药组成 ......
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