间皮瘤相关特异性抗体的研究进展
作者：邓群益
单位：山东省单县中心医院呼吸科 274300
关键词：
诊断病理学杂志000127分类号：R730.262 文献标识码：A
文章编号：1007-8096(2000)01-0067-03 自1979年Singh等[1]用培养的人反应性间皮细胞悬液作免疫原制备多克隆抗间皮细胞抗体以来，陆续用人恶性间皮瘤(MM)细胞株研制了MAb45，anti-MS和ME1等抗体。另外，识别卵巢肿瘤抗原的抗体OV632和K1及抗维尔姆斯瘤抗体anfi-WF1，在MM中亦有较高的敏感性和特异性。但它们或仅适用于冷冻组织，或是对MM缺乏完全特异性的细胞，故不适于实际诊断工作。最近发现几种针对间皮瘤相关抗原的抗体具有潜在的临床应用前景，见附表。
1MS-2761
, http://www.100md.com
是以培养的非肿瘤性间皮细胞为免疫原制备的单克隆抗体，它所识别的抗原为血管细胞粘附分子(VCAM-1)。Ya-mada等[2]研究表明，培养的间皮细胞MS-2761全部阳性，12例丙酮固定，石蜡包埋的胸膜间皮瘤亦全部呈阳性反应，但着色部位不同，MS-2761在良性纤维型间皮瘤细胞表面着色，在纤维基质中形成网状。在上皮样型或混合型MM的上皮样成分中，MS-2761着色于腺体样腔的内表面或肿瘤细胞胞浆，而肉瘤样MM染色强度弱于上皮成分，而且仅为胞浆着色。78例上皮肿瘤组织中MS-2761阴性77例，阴性率达99%，34例上皮肿瘤细胞株有33例阴性，阴性率达97%。
2HBME-1
是目前临床上广泛应用的间皮瘤相关抗体，它是用人上皮型MM细胞悬液作免疫原制备的单克隆抗体，可与存在于间皮瘤细胞微绒毛表面的抗原起反应。这种抗体可应用于常规固定，石蜡包埋标本，在大多数上皮型MM中呈强阳性反应，阳性细胞呈厚的周边型毛刷样，而低分化上皮型MM及肉瘤样型MM呈阴性反应，其它组织来源的腺癌和其它肿瘤亦可呈阳性反应[3]。Attanoos等[4]报道HBME-1在肺腺癌的阳性率高达72%。因此，许多作者认为HBME-1在区分上(全文见PDF)
, 百拇医药
参考文献：
[1]Singh G,Whiteside TL,Dekker A.Immunodiagmsis of mesothelioma:Use of antimesothelial cell serum in an indirect immunofluorescence assay.Cancer,1979,43:2288-2296
[2]Yamada T,Jiping J,Eado R,et al.Molecular cloning of a cell-surface gly-coprotein that can potentially discriminate mesothelium from epithelium:its identification as vascular cell adhesion molecule 1. Br J Cancer,1995,71:562-570
, 百拇医药
[3]Miettinen M, Kovatich AJ.HBME-1:a monoclclonal antibody useful in the differential diangnosis of mesothelioma,adenocarcinoma and soft tissue and bone tumor.Appl Immunohistochem, 1995,3:115-122
[4]Attanoos RL, Goddard H, Gibbs AR.Mesothelioma-binding antibody:thrombomodulin,OV632 and HBME-1 and their use in the diagnosis of malisnant mesothelioma.Histopathology,1996,29:209-215
[5]Ordonez NG.The Value of antibodies 44-3A6,SM3,HBME-1 and thrombomodulin in differentiating epithelial pleural mesothelioma from lung adencareinoma: comparative study with other commonly used anti-bodies. Am J Surg Pathol,1997,21:1399-1408
, 百拇医药
[6]Riera JR,Astengo-Osuna C,Longmate JA,et al.The immunohistochemical diagnostic panel for epithelial mesothelioma:a reevaluation after heat-in-duced epitope retrieval. Am J Surg Pathol,1997,21:1409-1419
[7]Donna A,Betta PG,Chiodera P,et al.Newly marketed tissue markers for malignant mesothelioma:immunoreactivity of rabbit AMAD-2 antiserum compared with monoclonalantibody HBME-1 and a review of the literature on so-called antimesothelioma antibodies.Hum Pathol,1997,28:929-937
, http://www.100md.com
[8]Collins CL,Ordonez NG,Schaefer R,et al. Thrombomodulin expression in malignant pleural mesothelioma and pulmonary adenocarcinoma. Am J Pathol,1992,141:827-833
[9]Brown RW,Clark GM,Tandon AK,et al.Multiple-marker immunohisto-chemical phenotypes distinsuishing malignant pleural mesothelioma from pulmonary adenocarcinoma. Hum Pathol,1993,24:347-354
[10]Doglioni C,Dei Tos AP,Laurino L,et al. Calretinin:a novel immunocy-tochemical marker for mesothelioma. Am J Surg Pathol,1996,20:1037-1046
, http://www.100md.com
[11]Kennedy AD,King G, Kerr KM.HBME-1 and antithrombomodulin in the differential diagnosis of malignant mesothelioma of pleura.J Clin Pathol 1997,50:859-862
[12]Leers MPG, Aarts MMJ, Theunissen PHMH. E-cadherin and calretinin: a useful combination of immunochemical markers for differentiation be-tween mesothelioma and metastatic adenocarcinoma.Histopathology,1998,32:209-216
[13]Soler AP,Knudsen A,Jaurand M-C,et al.The differential expression of N-cadherin and E-cadherin distinguishes pleural mesotheliomas from lung adenocarcinomas.Hum Pathol,1995,26:1363-1369
[14]Han AC,Soler AP, Knudsen KA,et al.Differential expression of N-cad-herin in pleural mesohliomsa and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues. Hum Pathol,1997,28:641-645
收稿日期：1999-03-30
修稿日期：1999-04-30, 百拇医药
单位：山东省单县中心医院呼吸科 274300
关键词：
诊断病理学杂志000127分类号：R730.262 文献标识码：A
文章编号：1007-8096(2000)01-0067-03 自1979年Singh等[1]用培养的人反应性间皮细胞悬液作免疫原制备多克隆抗间皮细胞抗体以来，陆续用人恶性间皮瘤(MM)细胞株研制了MAb45，anti-MS和ME1等抗体。另外，识别卵巢肿瘤抗原的抗体OV632和K1及抗维尔姆斯瘤抗体anfi-WF1，在MM中亦有较高的敏感性和特异性。但它们或仅适用于冷冻组织，或是对MM缺乏完全特异性的细胞，故不适于实际诊断工作。最近发现几种针对间皮瘤相关抗原的抗体具有潜在的临床应用前景，见附表。
1MS-2761
, http://www.100md.com
是以培养的非肿瘤性间皮细胞为免疫原制备的单克隆抗体，它所识别的抗原为血管细胞粘附分子(VCAM-1)。Ya-mada等[2]研究表明，培养的间皮细胞MS-2761全部阳性，12例丙酮固定，石蜡包埋的胸膜间皮瘤亦全部呈阳性反应，但着色部位不同，MS-2761在良性纤维型间皮瘤细胞表面着色，在纤维基质中形成网状。在上皮样型或混合型MM的上皮样成分中，MS-2761着色于腺体样腔的内表面或肿瘤细胞胞浆，而肉瘤样MM染色强度弱于上皮成分，而且仅为胞浆着色。78例上皮肿瘤组织中MS-2761阴性77例，阴性率达99%，34例上皮肿瘤细胞株有33例阴性，阴性率达97%。
2HBME-1
是目前临床上广泛应用的间皮瘤相关抗体，它是用人上皮型MM细胞悬液作免疫原制备的单克隆抗体，可与存在于间皮瘤细胞微绒毛表面的抗原起反应。这种抗体可应用于常规固定，石蜡包埋标本，在大多数上皮型MM中呈强阳性反应，阳性细胞呈厚的周边型毛刷样，而低分化上皮型MM及肉瘤样型MM呈阴性反应，其它组织来源的腺癌和其它肿瘤亦可呈阳性反应[3]。Attanoos等[4]报道HBME-1在肺腺癌的阳性率高达72%。因此，许多作者认为HBME-1在区分上(全文见PDF)
, 百拇医药
参考文献：
[1]Singh G,Whiteside TL,Dekker A.Immunodiagmsis of mesothelioma:Use of antimesothelial cell serum in an indirect immunofluorescence assay.Cancer,1979,43:2288-2296
[2]Yamada T,Jiping J,Eado R,et al.Molecular cloning of a cell-surface gly-coprotein that can potentially discriminate mesothelium from epithelium:its identification as vascular cell adhesion molecule 1. Br J Cancer,1995,71:562-570
, 百拇医药
[3]Miettinen M, Kovatich AJ.HBME-1:a monoclclonal antibody useful in the differential diangnosis of mesothelioma,adenocarcinoma and soft tissue and bone tumor.Appl Immunohistochem, 1995,3:115-122
[4]Attanoos RL, Goddard H, Gibbs AR.Mesothelioma-binding antibody:thrombomodulin,OV632 and HBME-1 and their use in the diagnosis of malisnant mesothelioma.Histopathology,1996,29:209-215
[5]Ordonez NG.The Value of antibodies 44-3A6,SM3,HBME-1 and thrombomodulin in differentiating epithelial pleural mesothelioma from lung adencareinoma: comparative study with other commonly used anti-bodies. Am J Surg Pathol,1997,21:1399-1408
, 百拇医药
[6]Riera JR,Astengo-Osuna C,Longmate JA,et al.The immunohistochemical diagnostic panel for epithelial mesothelioma:a reevaluation after heat-in-duced epitope retrieval. Am J Surg Pathol,1997,21:1409-1419
[7]Donna A,Betta PG,Chiodera P,et al.Newly marketed tissue markers for malignant mesothelioma:immunoreactivity of rabbit AMAD-2 antiserum compared with monoclonalantibody HBME-1 and a review of the literature on so-called antimesothelioma antibodies.Hum Pathol,1997,28:929-937
, http://www.100md.com
[8]Collins CL,Ordonez NG,Schaefer R,et al. Thrombomodulin expression in malignant pleural mesothelioma and pulmonary adenocarcinoma. Am J Pathol,1992,141:827-833
[9]Brown RW,Clark GM,Tandon AK,et al.Multiple-marker immunohisto-chemical phenotypes distinsuishing malignant pleural mesothelioma from pulmonary adenocarcinoma. Hum Pathol,1993,24:347-354
[10]Doglioni C,Dei Tos AP,Laurino L,et al. Calretinin:a novel immunocy-tochemical marker for mesothelioma. Am J Surg Pathol,1996,20:1037-1046
, http://www.100md.com
[11]Kennedy AD,King G, Kerr KM.HBME-1 and antithrombomodulin in the differential diagnosis of malignant mesothelioma of pleura.J Clin Pathol 1997,50:859-862
[12]Leers MPG, Aarts MMJ, Theunissen PHMH. E-cadherin and calretinin: a useful combination of immunochemical markers for differentiation be-tween mesothelioma and metastatic adenocarcinoma.Histopathology,1998,32:209-216
[13]Soler AP,Knudsen A,Jaurand M-C,et al.The differential expression of N-cadherin and E-cadherin distinguishes pleural mesotheliomas from lung adenocarcinomas.Hum Pathol,1995,26:1363-1369
[14]Han AC,Soler AP, Knudsen KA,et al.Differential expression of N-cad-herin in pleural mesohliomsa and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues. Hum Pathol,1997,28:641-645
收稿日期：1999-03-30
修稿日期：1999-04-30, 百拇医药