【摘要】乙型肝炎病毒是当今流行最广泛,危害最严重的病毒性肝炎之一，相当一部分乙肝携带者可以发展成慢性肝炎,需要接受病毒治疗。目前多以其血清感染标志物乙肝两对半及HBV-DNA定量，作为临床分析和判断乙肝患者病情、疗效及预后的主要依据,但由于两对半的组合模式复杂多样,导致部分患者的HBV感染复制状况难以判断,而HBV-DNA是乙肝病毒感染和复制的特异性指标,是表明其具有传染性的最有力的证据。但是受到实验室要求较高的严格限制,尚无法普及检测,为了进一步探讨乙肝两对半与HBV-DNA定量结果之间的关系。本文对ELISA法检测乙肝两对半与荧光定量PCR检测HBV-DNA的比较进行分析比较。

    【关键词】ELISA法检测；荧光定量PCR检测；HBV-DNA

    The ELISA law examination hepatitis B two fifty-fifty examine with fluorescence quota PCR to measure HBV-DNA the comparison

    QINMeiWENBo

    【Abstract】The hepatitis B virus is popular now widely, harms one of most serious toxic hepatitis, quite a part of hepatitis B carrier may develop the chronic hepatitis, needs to accept the viral treatment. At present are many by his/her the blood serum infection designated object hepatitis B two fifty-fifty and the HBV-DNA quota, takes the clinical analysis and the judgment hepatitis B patient condition, the curative effect and the prognosis main basis, but because two combination patterns fifty-fifty complex diverse, causes the partial patient's HBV infection duplication condition to judge with difficulty, but HBV-DNA is the hepatitis B virus infection and the duplication specificity target, is indicated that it has the infectious most strong evidence. But receives the laboratory request high strict limit, was still unable to popularize the examination, to further discuss the hepatitis B two fifty-fifty with the HBV-DNA quota result between relations. ......