针刺太冲和天枢对腹泻型肠易激综合征大鼠5—羟色胺、去甲肾上腺素及降钙素基因相关肽的影响(1)
摘要：目的 探讨电针不同穴位对腹泻型肠易激综合征(D-IBS)模型大鼠的效应差异。方法 采用急慢性应激刺激法制作D-IBS大鼠模型。将实验动物分为对照组、模型组、天枢+足三里组、太冲+足三里组，针刺组分别给予双侧相应穴位电针刺激20 min，隔日1次，共治疗7次。观察各组大鼠体征状况，检测各组大鼠血浆5-羟色胺(5-HT)、去甲肾上腺素(NE)、降钙素基因相关肽(CGRP)的含量。结果 与对照组比较，模型组大鼠血浆5-HT、NE含量明显增加，CGRP含量降低(P<0.05)；与模型组比较，针刺组大鼠5-HT、NE含量明显降低，CGRP含量升高(P<0.05)；两针刺组间比较无明显差异。结论 针刺“太冲”和“天枢”可能通过降低模型大鼠血浆5-HT、NE含量，增加CGRP含量达到治疗D-IBS的目的。
关键词：腹泻型肠易激综合征；电针；5-羟色胺；去甲肾上腺素；降钙素基因相关肽；大鼠
DOI：10.3969/j.issn.1005-5304.2015.10.015
中图分类号：R245 文献标识码：A 文章编号：1005-5304(2015)10-0050-04
Abstract：Objective To discuss the effects of electroacupuncture intervention in different acupoints of rats models with diarrhea type irritable bowel syndrome (D-IBS). Methods The rat models were established with acute and chronic stimulation. The rats were divided into the normal control group， model group， “Taichong” (LR3) + “Zusanli” (ST36) group， and “Tianshu” (ST25) + “Zusanli” (ST36) group. Electroacupuncture was applied to bilateral corresponding acupoints for 20 minutes， every other day for 7 times. The physical signs of each rat were observed. The contents of 5-HT， NE and CGRP in the rats’ plasma were detected by sandwich ELISA. Results Compared with the normal group， the contents of 5-HT and NE in the model group were obviously higher and the content of CGRP decreased (P<0.05). Compared with the model group， the contents of 5-HT and NE in all treatment groups were lower and the content of CGRP increased (P<0.05). There was no difference between the “Taichong” (LR3) + “Zusanli” (ST36) and “Tianshu” (ST25) + “Zusanli” (ST36) groups. Conclusion The mechanism of electroacupuncture may be realized through lowering the contents of 5-HT and NE in the model rats and increasing the content of CGRP to treat D-IBS.
Key words：D-IBS；electroacupuncture；5-HT；NE；CGRP；rats
肠易激综合征(irritable bowel syndrome，IBS)是临床中较常见的功能性肠道疾病，主要表现为腹痛、腹胀不适及大便次数增多、粪质稀薄等腹泻表现，或大便难排、粪质干结等便秘表现，或者腹泻与便秘交替出现等。其中以腹泻型肠易激综合征(diarrhea type irritable bowel syndrome，D-IBS)最为多见。研究表明，IBS发病主要是精神心理因素引起神经-内分泌-免疫网络调控失常引起。脑肠间的联系可能是通过神经肽类物质在神经系统及胃肠道效应细胞间传递而实现[1]。这些神经肽质不但存在于周围神经系统，也存在于中枢神经系统，各种脑肠肽间存在着相互作用，其中降钙素基因相关肽(CGRP)、5-羟色胺(5-HT)和去甲肾上腺素(NE)等脑肠肽在IBS发病中发挥着重要作用。本研究选用“太冲”+“足三里”、“天枢”+“足三里”，分别观察电针2组穴位对D-IBS大鼠血浆中CGRP、NE和5-HT的影响，探讨电针不同穴位对D-IBS模型大鼠的效应差异，为临床针刺治疗D-IBS提供实验依据。
1 材料与方法
1.1 动物与分组
SPF级健康Wistar大鼠60只，体质量(220±20)g，雌雄各半，山东鲁抗医药股份有限公司实验动物中心，许可证号SCXK(鲁)20130001。将动物平衡性饲养7 d，饲养环境温度为24～26 ℃，相对湿度45%～65%，通风良好。随机数字法将大鼠分为对照组、模型组、天枢+足三里组、太冲+足三里组，每组15只。
1.2 主要试剂与仪器 (卢岩等)
关键词：腹泻型肠易激综合征；电针；5-羟色胺；去甲肾上腺素；降钙素基因相关肽；大鼠
DOI：10.3969/j.issn.1005-5304.2015.10.015
中图分类号：R245 文献标识码：A 文章编号：1005-5304(2015)10-0050-04
Abstract：Objective To discuss the effects of electroacupuncture intervention in different acupoints of rats models with diarrhea type irritable bowel syndrome (D-IBS). Methods The rat models were established with acute and chronic stimulation. The rats were divided into the normal control group， model group， “Taichong” (LR3) + “Zusanli” (ST36) group， and “Tianshu” (ST25) + “Zusanli” (ST36) group. Electroacupuncture was applied to bilateral corresponding acupoints for 20 minutes， every other day for 7 times. The physical signs of each rat were observed. The contents of 5-HT， NE and CGRP in the rats’ plasma were detected by sandwich ELISA. Results Compared with the normal group， the contents of 5-HT and NE in the model group were obviously higher and the content of CGRP decreased (P<0.05). Compared with the model group， the contents of 5-HT and NE in all treatment groups were lower and the content of CGRP increased (P<0.05). There was no difference between the “Taichong” (LR3) + “Zusanli” (ST36) and “Tianshu” (ST25) + “Zusanli” (ST36) groups. Conclusion The mechanism of electroacupuncture may be realized through lowering the contents of 5-HT and NE in the model rats and increasing the content of CGRP to treat D-IBS.
Key words：D-IBS；electroacupuncture；5-HT；NE；CGRP；rats
肠易激综合征(irritable bowel syndrome，IBS)是临床中较常见的功能性肠道疾病，主要表现为腹痛、腹胀不适及大便次数增多、粪质稀薄等腹泻表现，或大便难排、粪质干结等便秘表现，或者腹泻与便秘交替出现等。其中以腹泻型肠易激综合征(diarrhea type irritable bowel syndrome，D-IBS)最为多见。研究表明，IBS发病主要是精神心理因素引起神经-内分泌-免疫网络调控失常引起。脑肠间的联系可能是通过神经肽类物质在神经系统及胃肠道效应细胞间传递而实现[1]。这些神经肽质不但存在于周围神经系统，也存在于中枢神经系统，各种脑肠肽间存在着相互作用，其中降钙素基因相关肽(CGRP)、5-羟色胺(5-HT)和去甲肾上腺素(NE)等脑肠肽在IBS发病中发挥着重要作用。本研究选用“太冲”+“足三里”、“天枢”+“足三里”，分别观察电针2组穴位对D-IBS大鼠血浆中CGRP、NE和5-HT的影响，探讨电针不同穴位对D-IBS模型大鼠的效应差异，为临床针刺治疗D-IBS提供实验依据。
1 材料与方法
1.1 动物与分组
SPF级健康Wistar大鼠60只，体质量(220±20)g，雌雄各半，山东鲁抗医药股份有限公司实验动物中心，许可证号SCXK(鲁)20130001。将动物平衡性饲养7 d，饲养环境温度为24～26 ℃，相对湿度45%～65%，通风良好。随机数字法将大鼠分为对照组、模型组、天枢+足三里组、太冲+足三里组，每组15只。
1.2 主要试剂与仪器 (卢岩等)