乙型肝炎病毒基因亚型及相关指标临床意义分析(1)
摘要：目的 探讨乙型肝炎病毒不同基因亚型与病毒复制状况、凝血功能、肝脏功能的关系，以及不同基因亚型的临床意义。方法 选取慢性乙型肝炎、肝硬化、肝癌患者血液标本，检测乙型肝炎病毒基因亚型、病毒载量、凝血功能、肝功能等指标，应用统计软件SPSS 16.0进行分析。结果 本次调查乙型肝炎病毒基因亚型主要为B型和C型，B型略多于C型。但在肝癌患者中，C型所占比例高于B型(P<0.05)。C型患者病毒载量高于B型(P<0.05)。不同基因亚型患者凝血功能指标差异有统计学意义(P<0.05)，肝功能差异无统计学意义(P>0.05)。结论 本地区乙型肝炎病毒基因亚型主要为B型和C型，C型患者肝脏损害较B型重，提示不同基因亚型可能影响患者体内病毒复制，并引起不同临床损害程度的慢性肝病。
关键词：乙型肝炎病毒；基因型
Clinical Correlation Research of Hepatitis B Virus Genotypes
, http://www.100md.com
HUANG Bo， WAN Xiao-tao， ZHANG You-hui，ZHONG Fang-cai
(Department of Clinical Laboratory，The First People's Hospital of Neijiang， Neijiang 641000，Sichuan，China)
Abstract：Objective To understand the distribution of genotypes in Neijiang area， analyze the relationship of different genotypes with virus replication， liver function and coagulation function， investigate the clinical significance of different genotypes. Methods Serum samples were collected from chronic hepatitis B， liver cirrhosis， hepatic cell carcinoma individuals. Hepatitis B virus genotypes， virus replication， liver function and coagulation function were detected. The data was analyzed with SPSS 16.0.Results The main genotypes in Neijiang area were B and C. The C genotype virus replication was significantly higher than that of B genotype(P﹤0.05).The proportion of C genotype in hepatic cell carcinoma group exhibited significantly higher than that in B genotype(P﹤0.05).No significant difference was found in liver function between B and C genotypes(P>0.05). But significant difference was found in coagulation function between B and C genotypes(P﹤0.05). Conclusion The main genotypes in Neijiang area were B and C. The liver damage of patients with C genotype is more serious than that of B genotype， which prompts that hepatitis B virus genotypes may influence on in vivo hepatitis B virus replication and may cause chronic liver disease of different clinical types.
, 百拇医药
Key words：Hepatitis B virus；Genotype
病毒性肝炎是严重危害人类健康的疾病，其中乙型病毒肝炎是危害较广泛的慢性传染病，目前全世界大概有近20亿人感染过乙型肝炎病毒，其中有超过3亿人发展为慢性感染者。我国是乙型肝炎病毒感染的高发区，人群中乙型肝炎病毒的感染率高，乙型肝炎表面抗原阳性率高达7.15%。乙型肝炎病毒感染人体后，损伤肝细胞，严重者导致肝细胞坏死，少数发展为肝硬化乃至肝癌，预后较差，迁延不愈，严重危害人类生命健康。乙型肝炎病毒感染人体后的临床状况、治疗效果、预后等与病毒的不同基因亚型都有一定的关系[1]。为探讨本地区乙肝患者基因亚型和相关指标的关系及临床意义，本调查选取了慢性乙型肝炎、肝硬化及肝癌患者血液标本，检测乙肝病毒基因亚型及病毒载量、凝血功能、肝功能等指标，现报道如下。
1 资料与方法
1.1一般资料 标本来源于2014年9月～2015年7月在本医院住院患者，经检测乙肝表面抗原阳性且血清HBV-DNA拷贝数≥1×103患者172例，其中男性105例，女性67例，年龄16～70岁。所有病例排除合并其它肝炎病毒感染、脂肪肝、酒精肝、原发性胆汁性肝硬化及自身免疫性肝炎等，均未使用溶栓及抗凝药物。其中慢性乙型肝炎98例，肝硬化40例，肝癌34例，全部按照慢性乙型肝炎诊断标准[2]。标本均为早晨空腹静脉血，当天及时分离血清并检测。, http://www.100md.com(黄波 万小涛 张有辉 钟方财)
关键词：乙型肝炎病毒；基因型
Clinical Correlation Research of Hepatitis B Virus Genotypes
, http://www.100md.com
HUANG Bo， WAN Xiao-tao， ZHANG You-hui，ZHONG Fang-cai
(Department of Clinical Laboratory，The First People's Hospital of Neijiang， Neijiang 641000，Sichuan，China)
Abstract：Objective To understand the distribution of genotypes in Neijiang area， analyze the relationship of different genotypes with virus replication， liver function and coagulation function， investigate the clinical significance of different genotypes. Methods Serum samples were collected from chronic hepatitis B， liver cirrhosis， hepatic cell carcinoma individuals. Hepatitis B virus genotypes， virus replication， liver function and coagulation function were detected. The data was analyzed with SPSS 16.0.Results The main genotypes in Neijiang area were B and C. The C genotype virus replication was significantly higher than that of B genotype(P﹤0.05).The proportion of C genotype in hepatic cell carcinoma group exhibited significantly higher than that in B genotype(P﹤0.05).No significant difference was found in liver function between B and C genotypes(P>0.05). But significant difference was found in coagulation function between B and C genotypes(P﹤0.05). Conclusion The main genotypes in Neijiang area were B and C. The liver damage of patients with C genotype is more serious than that of B genotype， which prompts that hepatitis B virus genotypes may influence on in vivo hepatitis B virus replication and may cause chronic liver disease of different clinical types.
, 百拇医药
Key words：Hepatitis B virus；Genotype
病毒性肝炎是严重危害人类健康的疾病，其中乙型病毒肝炎是危害较广泛的慢性传染病，目前全世界大概有近20亿人感染过乙型肝炎病毒，其中有超过3亿人发展为慢性感染者。我国是乙型肝炎病毒感染的高发区，人群中乙型肝炎病毒的感染率高，乙型肝炎表面抗原阳性率高达7.15%。乙型肝炎病毒感染人体后，损伤肝细胞，严重者导致肝细胞坏死，少数发展为肝硬化乃至肝癌，预后较差，迁延不愈，严重危害人类生命健康。乙型肝炎病毒感染人体后的临床状况、治疗效果、预后等与病毒的不同基因亚型都有一定的关系[1]。为探讨本地区乙肝患者基因亚型和相关指标的关系及临床意义，本调查选取了慢性乙型肝炎、肝硬化及肝癌患者血液标本，检测乙肝病毒基因亚型及病毒载量、凝血功能、肝功能等指标，现报道如下。
1 资料与方法
1.1一般资料 标本来源于2014年9月～2015年7月在本医院住院患者，经检测乙肝表面抗原阳性且血清HBV-DNA拷贝数≥1×103患者172例，其中男性105例，女性67例，年龄16～70岁。所有病例排除合并其它肝炎病毒感染、脂肪肝、酒精肝、原发性胆汁性肝硬化及自身免疫性肝炎等，均未使用溶栓及抗凝药物。其中慢性乙型肝炎98例，肝硬化40例，肝癌34例，全部按照慢性乙型肝炎诊断标准[2]。标本均为早晨空腹静脉血，当天及时分离血清并检测。, http://www.100md.com(黄波 万小涛 张有辉 钟方财)