SDF-1对创缘表皮干细胞定向趋化及促创面愈合效应的研究(1)
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[摘要]目的:观察干细胞趋化因子SDF-1在创面愈合过程中调控表皮干细胞定向迁移促进创面愈合的过程。方法:制作大鼠背部全层皮肤缺损模型,随机分为三个实验组:①SDF-1组;②AMD3100(CXCR4受体的拮抗剂)组;③空白对照组。分别于致伤后1天、3天、5天、7天和12天照相计算伤后不同时间创面占初始创面的百分比并取材。运用HE染色和免疫组化技术观察创缘表皮干细胞定向迁移分化的特点,寻找其规律。结果:与其他两个处理组相比,SDF-1处理组愈合时间缩短,其创缘皮表皮基底层细胞分裂增殖旺盛,创缘表皮干细胞数目明显多于其他两组。结论:在创面愈合过程中,SDF-1可以调控表皮干细胞向创缘迁移,加速创面上皮化。
[关键词]基质细胞衍生因子-1;表皮干细胞;创面愈合
[中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455(2010)08-1156-05
SDF-1 accelerates wound healing via recruitment of epidermal stem cells
ZHANG Yi1,GONG Xi-yuan1,SUN Xue-wu1,QIU Xiao-dong1,GUO Dan-feng1,ZHANG Guan-jun1,HAN Ji-qin1,CAO Chuan2
(1.Department of Plastic Surgery,Jinan Central Hospital of Shandong University,Jinan 200013,China; 2.Department of Plastic Surgery,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)
Abstract:ObjectiveTo investigate the stem cell chemokine SDF-1 in the regulation of wound healing migration of epidermal stem cells to promote wound healing process.MethodsRat dorsal full-thickness skin defects were randomly divided into three experimental groups: ①SDF-1 group; ②AMD3100 (CXCR4 receptor antagonist) group;③control group. 1 day,3 days,5 days,7 days and 12 days after injury camera calculates the wound at different times,and total percentage of the initial wound drawn. Using HE staining and immunohistochemical observation of a margin of epidermal migration and differentiation of stem cell characteristics,to find its own rules. ResultsCompared with the other two treatment groups,SDF-1 treatment to shorten healing time,the margin of skin epidermal basal cell division and proliferative margin of the number of epidermal stem cells was more than the other two groups.ConclusionIn the wound healing process,SDF-1 can regulate epidermal stem cells migrate to the wound edge,accelerate wound epithelization.
Key words:SDF-1; epidermal stem cells; wound healing
位于表皮基底层的表皮干细胞是皮肤组织发生、创面修复与改建的关键细胞[1-2]。β1整合素和角蛋白19(Keratin-19 K19)染色呈阳性为其鉴别手段[3-4]。研究发现,创面修复过程中表皮干细胞可向创缘定向迁移[5]。理论上讲,这种迁移趋势是由趋化因子与其靶细胞膜上特异性受体结合,从而调控这些细胞的迁移、增殖和分化来发挥促修复作用。基质细胞衍生因子-1(stromal cellderived factor-1 SDF-1)和其受体CXCR4广泛表达于多种组织细胞,对一些组织器官发育和损伤后修复再生起重要调节作用 ......
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