补肾复方对压力负荷增加大鼠心肌纤维化的影响(1)
 |
| 第1页 |
参见附件。
摘 要:目的:观察补肾复方逆转压力负荷增加大鼠心肌纤维化的作用。方法:采用腹主动脉狭窄所致压力负荷增加大鼠左室重构模型,将雄性SD大鼠60只随机分为假手术组、模型组、卡托普利组、补肾复方大剂量组、补肾复方小剂量组,观察用药4周后左室心肌病理形态胶原染色、左室心肌间质超微结构、左室心肌Ⅰ型胶原免疫组织化学染色、左室心肌Ⅲ型胶原免疫组织化学染色等指标的改变。结果:模型组Ⅰ型胶原的平均光密度与假手术组比显著升高(P<0.01),卡托普利组、补肾复方大剂量组、小剂量组的Ⅰ型胶原平均光密度均较模型组显著下降(P<0.01)。模型组Ⅲ型胶原的平均光密度与假手术组比显著升高(P<0.01),卡托普利组、补肾复方大剂量组、小剂量组的Ⅲ型胶原平均光密度均较模型组显著下降(P<0.01)。左室心肌病理形态胶原染色、左室心肌间质超微结构的改变与Ⅰ型胶原和Ⅲ型胶原的改变基本一致。结论:补肾复方具有与卡托普利基本一致的逆转心肌纤维化的作用。
关键词:心肌纤维化;压力负荷;Ⅰ型和Ⅲ型胶原;补肾复方
中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)04-0677-05
Study on Regressing Myocardial Fibrosis in Rat with Pressure
Overload by Bu Shen Prescription
CAI Hui, ZHANG Qunyan, DONG Xiaolei,ZHAO Zhiming,GUO Junhao,SHANG Wei
(Department of Integrated Traditional Chinese and Western Medicine, Nanjing General Hospital of Nanjing Military Command, PLA, Nanjing 210002, Jiangsu)
Abstract:Objective:To observe the effects of Bu Shen Prescription on regressing myocardial fibrosis in rat with pressure overload. Methods:The model of left ventricular remodeling in rat with pressure overload by abdominal aortic constriction was used. 60 SD male rats were randomly divided into five groups: sham operation group, model group, captopril group, Bu Shen Prescription high dosage group and low dosage group. After treatment 4 weeks, it was observed that the changes of left ventricular myocardium collagen pathological coloring, left ventricular interstitial cardiac tissue ultramicrostructure, left ventricular cardiac tissue collagen type Ⅰimmunohistochemistry coloring and collagen type Ⅲ immunohistochemistry coloring. Results: The average optic density of left ventricular cardiac tissue type Ⅰcollagen and type Ⅲ collagen of model group were increased obviously than that of sham operation group (P<0.01). The average optic density of left ventricular cardiac tissue type Ⅰcollagen and type Ⅲ collagen of captopril group, Bu Shen Prescription high dosage group and low dosage group were lower than those of model group significantly(P<0.01). The changes of left ventricular myocardium collagen pathological coloring and left ventricular interstitial cardiac tissue ultramicrostructure were consistent with those of typeⅠ collagen and type Ⅲ collagen ......
您现在查看是摘要介绍页，详见PDF附件(3637kb)。