丙型肝炎病毒抗体检测结果分析
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[摘要] 目的：分析HCV抗体检测结果并探讨解决问题的对策。方法：结合全国血站系统室间质评，对我站2009年ELISA方法检测HCV抗体的结果进行分析。结果：我站2009年共采集血液24 569 U(200 ml为1 U)，ELISA方法检测HCV抗体报废360 U，其中阳性报废162 U，可疑报废198 U，可疑报废占HCV总报废比为55.00%。2005年血站系统第3次室间质评0574号标本回报结果准确率为35.51%。结论：血站HCV检测还存在假阳性和假阴性的问题，提高试剂质量和改进检测方法可以更有效地减少血液浪费，保证血液安全。
[关键词] HCV抗体；ELISA；检测；分析
[中图分类号] R446.6 [文献标识码] B[文章编号] 1673-7210(2010)10(a)-074-02
The analysis of HCV-Ab detection results
LI Jia
(The Central Blood Bank of Dandong City, Liaoning Province, Dandong 118002)
[Abstract] Objective: To analyze the results of HCV-Ab test and discuss the countermeasure of problem-solving. Methods: The results of HCV-Ab detected by ELISA in 2009 linking with the external quality assessment of nationwide blood bank were analyzed. Results: 24 569 U(200 ml=1 U) blood were collected in 2009 and 360 U blood were abandoned altogether owing to HCV-Ab by ELISA, among reported 162 U as positive, reported 178 U as suspect. The rate as suspect was 55.00%. The accuracy rate of 0574's result was 35.51% in the third time external quality assessment of blood bank in 2005. Conclusion: The HCV-Ab test exists false positive and false negative in blood bank. Testers should reduce waste and insure safety of blood by improving the reagent quality and the test method ......
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