论文下载：丹酚酸B对大鼠非酒精性脂肪性肝炎的影响(论著)

丹酚酸B对大鼠非酒精性脂肪性肝炎的影响(1)

http://www.100md.com 2010年11月25日王迎春刘炎芳朱瑞萍

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     【摘要】目的探讨丹酚酸B对大鼠非酒精性脂肪性肝炎的疗效。方法雄性SD大鼠36只随机分组,对照组、模型组及丹酚酸B治疗组各12只,对照组给予普通饲料,其余各组均给予高脂饲料。于12周处死模型组。治疗组每天以浓度为1 mg/ml的丹酚酸B溶液20 ml/kg灌胃,同时继续高脂饲料喂养,对照组以20 ml/kg蒸馏水灌胃,同时继续普通饲料喂养。于实验第24周处死剩余实验动物。测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、三酰甘油(TG)、总胆固醇(TC)及肝组织丙二醛(MDA)含量,同时观察肝组织病理学改变,免疫组化法检测肝组织TIMP1的表达,RTPCR法检测肝组织TGFβ1mRNA的表达。结果与正常对照组相比,模型组肝指数、血清ALT、AST、TG、TC及肝组织MDA水平显著升高(P<001),肝组织炎症活动度计分、TIMP1及TGFβ1mRNA的表达均显著升高(P<001)。丹酚酸B治疗组血清ALT、AST、TG、TC及肝组织MDA水平较模型组显著降低(P<001),肝组织炎症活动度计分、TIMP1及TGFβ1mRNA的表达均较模型组显著降低(P<001 或P<005)。结论丹酚酸B对高脂饮食大鼠非酒精性脂肪性肝炎有一定的治疗作用。

    【关键词】

    丹酚酸B;大鼠;非酒精性脂肪性肝炎

    Effects of Salvianolic acid B on nonalcoholic stentohepatitis in rats

    WANG Yingchun, LIU Yanfang, ZHU Ruiping Department of Gastroenterology, Sun Yatsen Hospital of Dalian University, DaLian 116001, China

    

    【Abstract】 Objective To study the therapeutic role of Salvianolic acid B(SAB)in treating nonalcoholic stentohepatitis(NASH)in rats Methods Thirtysix male SD rats were randomly divided into 3 groups The normal control group (n=12)wse fed with normal diet.NASH model group (n=12)and therapeutic group(n=12)were fed with fat rich diet, The rats of NASH model group were killed at the 12 th week Therapeutic group was treated with SAB at the dose of 10 mg/kg from the 13 th week and finished at the 24 th week The serum ALT, AST, TG, TC and liver tissue MOD levels were measured, The degrees of hepatic histologic change were observed with HE, Tissue inhibitor of metalloproteinase one(TIMP1) was examined with immunohistochemistry, And the gene mRNA level of transforming growth factor beta one(TGFβ1) was deteced with reverse transcription polymerase chain reaction(RTPCR)Results Compared with the normal control group, the liver index, the serum AIT, AST, TG, TC and liver tissue MOD levels were increased significantly(P<001)in NASH model control group.The histological activity index(HAI) score, expressions of TIMP1and TGFβ1mRNA were increased significantly(P<001) Compared with NASH modeI control group, The above indexes in the therapeutic group were decreased significantly(P<005 or P<001)Conclusion SAB has some therapeutic role in treating NASH of rats ......

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