拜阿司匹灵预处理对高血糖SD大鼠局部脑缺血再灌注损伤中ICAM 1的影响(1)
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【摘要】 目的 采用高血糖条件下SD大鼠局灶性脑缺血再灌注损伤模型,通过观察大鼠脑梗死体积、细胞间粘附分子的表达情况,探讨预防性应用拜阿司匹灵对高血糖条件下SD大鼠局灶性脑缺血再灌注损伤的脑保护作用。方法 随机分为对照组与拜阿司匹灵组,两组按缺血90 min再灌注3 h、6 h、12 h再分为3个亚组。各组均建立高血糖模型及SD大鼠右侧大脑中动脉缺血再灌注模型。分别测量梗死体积,免疫组化方法测定ICAM 1的表达情况。结果 拜阿司匹灵组与对照组相同再灌注时间点相比较:梗死体积减小(P均<0.01);缺血区ICAM 1表达减少(P均<0.01)。结论 预防性应用拜阿司匹灵能减轻高血糖条件下的局灶性脑缺血再灌注损伤,缩小梗死体积,使损伤脑区ICAM 1表达减少。
【关键词】拜阿司匹灵;高血糖;脑缺血再灌注;ICAM 1
Effect of bay aspirin on ICAM 1 on focal cerebral ischemia reperfusion damage in SD rat with hyperlycemia
SONG Ni Na,GAO Zheng,SUN Wen Fang,et al.The North Hospital of Dalian Medical University No.2 Affiliated Hospital,Dalian 116031,China
【Abstract】 Objective Applying focal ischemia reperfusion model of SD rat on the condition of hyperglycemia,to obserrve cerebral infarction volume of rat and expression of intercellular adhesion molecule 1 to discuss protective effect of pretreatment ofbay aspirin on the condition of hyperglycemia.Methods SD rat were randomly divided into two groups,control group and bay aspirin group.Each groupwas divided into three subgroups according to reperfusion 3 h、6 h and 24 h after ischemia for 90 minutes.Establish hyperglycemia model and ischemia reperfusion model in right cerebral middle artery of SD rat.Measuring the infarction volume and observing the expression of ICAM 1 with immunohistological method.Results Comparison between bay aspirin group and control group at the same point of time,infarction volume decreased,P<0.01,and ICAM 1 at the ischemia area decreased,P<0.01.Conclusion Under condition of hyperglycemia,preventive pretreatment by bay aspirin can decrease focal ischemia reperfusion damage,reduce infarction volume,reduce the expression of ICAM 1.
【Key words】 Bay aspirin; Ischemia reperfusion; Hyperglycemia; ICAM 1
脑缺血再灌注损伤是目前治疗缺血性脑血管病的靶点和热点问题,缺血性脑血管病急性期的高血糖使其临床转归更差。阿司匹林在缺血性脑血管病的治疗及二级预防中的保护作用已得到肯定,对其作用机制的研究也取得较大进展;但其一级预防作用尚未肯定,特别是在高血糖情况下其一级预防作用,至今尚无研究。本研究旨在通过预防性应用拜阿司匹灵,观察在高血糖状态下,SD大鼠脑缺血再灌注后脑梗死体积、细胞间粘附分子 1(ICAM 1)的表达情况,并探讨高血糖状态下,拜阿司匹灵预处理后对大鼠脑缺血再灌注损伤是否有保护作用。
1 材料与方法
1.1 实验动物及分组 健康雄性Sprague Dawley(SD)大鼠共36只,体质量180~220 g,血糖正常(〈6 mmol/l〉,术前禁食12 h ......
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