分娩方式对新生儿脐血HMGB1和Hs-CRP影响的研究(1)
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【摘要】 目的 了解不同分娩方式对新生儿脐血高迁移率蛋白-1(HMGB1)和高敏CRP(Hs-CRP)水平的影响。方法 随机选择住院分娩、无妊娠合并症及并发症的产妇120例为研究对象,按分娩方式分为经阴道分娩组和择期剖宫产组,于分娩断脐后采集脐动脉血测定HMGB1和Hs-CRP含量。结果 阴道分娩组脐动脉血HMGB1和Hs-CRP含量明显高于剖宫产组,且存在显著性差异(t=8.36,7.29,P<0.05);同时HMGB1和Hs-CRP之间存在显著正相关(r=0.63,P<0.01)。结论 经阴道分娩时宫缩及产道挤压使胎儿HMGB1和Hs-CRP水平升高。
【关键词】 分娩方式;新生儿;脐血; HMGB1;高敏CRP(Hs-CRP)
Study on influences of delivery patterns on HMGB1 and high-sensitive C-reactive protein in newborn cord blood
LI Fang-ying,HUA Qian,BAN Kai-bin,et al.The Central People’s Hospital of Tengzhou, Shandong 277500,China
【Abstract】 Objective To investigate influences of delivery patterns on levels of high mobility group box-1 (HMGB1) and high-sensitive C-reactive protein(Hs-CRP) in cord blood of the newborn.Methods 120 parturients without any pregnancy associated diseases and complications and without fetal distress,neonatal malformation divided into normal delivery group and selected cesarean section group according to their delivery patterns.The umbilical arterial blood samples were taken immediately after cutting the umbilical cord from placenta and the levels of HMGB1 and Hs-CRP were detected.Results The level of HMGB1 in normal delivery group was higer than that selected cesarean section group,there was significant difference between the two groups(t=8.36,P<0.05).The level of Hs-CRP in normal delivery group was higer than that selected cesarean section group,there was significant difference between the two groups(t=7.29,P<0.05).There were a correlation between HMGB1 and Hs-CRP (r=0.63,P<0.01).Conclusion The uterine contraction and obstetric canal squeeze on the fetus during normal vaginal delivery enable levels of HMGB1 and Hs-CRP in the cord blood of the newborn.
【Key words】Delivery pattern; The newborn; Cord blood; High mobility group box-1(HMGB1);High-sensitive C-reactive protein(Hs-CRP)
高迁移率族蛋白-1(HMGB1)是近年发现的一种重要的晚期炎症介质,在炎症反应过程中表达升高较晚,维持时间较长,与组织损伤后修复、炎症反应等方面关系密切[1]。为探讨不同分娩方式与HMGB1水平的关系,我们检测了经阴道分娩、剖宫产分娩两种不同分娩方式的新生儿脐血HMGB1及高敏CRP(Hs-CRP)含量,探讨不同分娩方式与新生儿对外界刺激反应的不同及其可能机制,为提倡阴道分娩、降低剖宫产率,提供理论依据。
1 资料与方法
1.1 研究对象 选择我院2009年7月至2010年5月足月单胎分娩产妇,年龄22~34岁,其中经阴道顺产60例,足月择期剖宫产60例,新生儿评分为8~10分,体质量≥2500 g,两组孕妇均身体健康无妊娠期合并症、并发症等疾病,妊娠期间无用药史、选择性剖宫产指征为社会因素、前置胎盘、骨盆异常、巨大儿、臀位、胎膜早破、所有新生儿均无窒息、宫内感染及先天畸形,且两组新生儿在性别、体重等方面不存在差异性。
1.2 方法
1.2.1 标本采集与制备 所有新生儿出生后在第一次哭叫前立即钳夹约20 cm长的脐带-将所取脐带一端的脐静脉以止血钳夹闭,用注射器抽取4 ml脐动脉血,3000 r/min离心10 min,分离出血清放入-70℃冰箱保存待检 ......
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