肾脏缺血后处理对兔急性肠道缺血再灌注损伤的保护作用(1)
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【摘要】 目的 探讨肾脏缺血后处理对兔急性肠道缺血再灌注损伤的保护作用及其可能的机制。方法 健康新西兰大白兔30只,随机分为3组(每组10只):对照组(Con)、肠道缺血后处理(IIP)、肾脏缺血后处理(RIP)。再灌注结束后测定血浆中丙二醛(MDA)含量;实验结束后光镜下观察肠道组织形态学变化以及测定肠道组织髓过氧化物酶(MPO)活性。结果 IIP和RIP组再灌注2 h后MDA的含量明显的低于Con组(P<0.01); SIP和RIP组再灌注2 h后组织MPO的活性明显的低于Con组(P<0.01); SIP和RIP组再灌注2 h后光镜下观察肠道组织形态学变化证实肠道损伤程度的明显的轻于Con组(P<0.01)。结论 肠道缺血再灌注前肾脏短暂缺血再灌注对肠道缺血再灌注损伤具有显著保护作用。这种远离器官缺血后处理对肠道保护作用可能与减轻活性氧的损伤及抗氧化作用加强有关。
【关键词】 远隔缺血后处理;缺血再灌注;急性肠缺血
Impact of remote postconditioning on rabbits with acute intestinal ischemia-reperfusion injury
CHUYong-xin, YANGMu, ZHANGju-wen.
Department of Vascular surgery,Yantai Yuhuangding Hospital,the Affiliated Hospital of medical College,Qingdao University,Shandong 264000,China
【Abstract】 Objective In this study, we tested the hypothesis that remote postconditioning induced by a single 5-min episode of renal artery occlusion and reperfusion applied immediately before the onset ofsuperior mesenteric artery reperfusion protects the intestinal from reperfusion injury. Methods 30 healthy New Zealand white rabbits were randomly divided into three groups(n=10 in each group): control, intestinal ischemic postconditioning (IIP), and renal ischemic postconditioning (RIP). Tissue myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels were determined and the morphology of some intestinal tissues was observed by microscopy at the end of the experiment. Results MDA levels and MPO activity was significantly reduced in IIP and RIP as compared to control (P<0.01),also was confirmed by the score of intestinal mucosal injury. Conclusion Renal postconditioning provides potent intestinal mucosal injury. The potential mechanism of remote postconditioning might be associated decreasing the injury caused by oxygen free radicals and strengthening the action of antioxidation.
【Key words】 Remote postconditioning;Ischemia - reperfusion injury;Acute intestinal ischemia
DOI:10.3760/cma.j.issn 1673-8799.2010.11.46
作者单位:264000青岛大学医学院附属烟台毓璜顶医院血管外科
越来越多的肠系膜上动脉栓塞和血栓形成早期发现早期手术治疗肠缺血再灌注损伤越来越受到临床医生的重视。缺血预处理和缺血后适应均可减轻缺血再灌注的损伤。但无法预知组织及器官缺血具体时间,缺血预处理的临床应用受到了极大限制。缺血后适应的实施直接作用于已发生缺血的重要器官,在临床上不适用于对缺血极其敏感的器官 ......
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