HPLC法同时测定不同产地甘草药材中甘草酸和甘草苷的含量
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【摘要】目的：测定不同产地甘草药材中甘草酸和甘草苷的含量。方法：采用HPLC法，检测波长为237 nm，流动相为0.05%磷酸溶液-乙腈。结果：甘草苷和甘草酸的线性范围分别为0.050~ 0.375 μg和0.40~3.00 μg，平均回收率分别为100.15%和98.76%。结论：所建HPLC方法操作简便、准确、重复性好，可为不同产地甘草药材的质量评价提供依据。
【关键词】高效液相色谱法；甘草；甘草酸；甘草苷；含量测定
Determination of glycyrrhizic acid and liquiritin in
Glycyrrhizae radix et rhizoma by HPLC
Li Ru-Jie
(Pharmacy of Sichuan Orthopaedic Hospital, Chengdu 610041, China)
【Abstract】Objective: To establish an HPLC method for determining the contents of glycyrrhizic acid and liquiritin in Glycyrrhizae radix et rhizoma. Methods: The mobile phase was acetonitrile and water containing 0.05% phosphoric acid. The detection wavelength was set at 237nm. Results: The linear range of glycyrrhizic acid and liquiritin was 0.40~3.00 μg, 0.050~ 0.375 μg and the average recovery was 98.76%, 100.15%, respectively. Conclusion: The developed method is simple, accurate, and can provide a scientific basis for the quality evaluation of Glycyrrhizae radix et rhizoma.
【Key words】HPLC; Glycyrrhizae radix et rhizoma; Glycyrrhizic acid; Liquiritin; Content determination
【中图分类号】R285 ......
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