食管鳞癌组织中线粒体和细胞核DNA的提取和鉴定
食管肿瘤;鳞状细胞癌;线粒体DNA;核DNA;提取,刘宗文,赵秋民,赵志华,李晟磊,庞霞,高冬玲,陈奎生,张云汉,通讯作者:,ExtractionandidentificationofmitochondrialDNAandnuclearDNAfro
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刘宗文, 赵秋民, 赵志华, 李晟磊, 庞霞, 高冬玲, 陈奎生, 张云汉, 河南省肿瘤病理重点实验室, 郑州大学第一附属医院病理科 河南省郑州市 450052
“十五”“211工程”重点学科建设项目, 教重办(2002)第2号
通讯作者: 张云汉, 450052, 河南省郑州市, 河南省肿瘤病理重点实验室, 郑州大学第一附属医院病理科. zhangyh@zzu.edu.cn
收稿日期: 2007-01-14 接受日期: 2007-01-27
Extraction and identification of mitochondrial DNA and nuclear DNA from human esophageal squamous cell carcinoma tissues
Zong-Wen Liu, Qiu-Min Zhao, Zhi-Hua Zhao, Sheng-Lei Li, Xia Pang, Dong-Ling Gao, Kui-Sheng Chen, Yun-Han Zhang
Zong-Wen Liu, Qiu-Min Zhao, Zhi-Hua Zhao, Sheng-Lei Li, Xia Pang, Dong-Ling Gao, Kui-Sheng Chen, Yun-Han Zhang,He’nan Key Laboratory of Tumor Pathology; Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, He’nan Province, China
Supported by theTenth Five-Year Plan Research Foundation for the Key Construction Project (211 Projects) of Zhengzhou University, No. 2002-2
Correspondence to:Yun-Han Zhang, He’nan Key Laboratory of Tumor Pathology; Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, He’nan Province, China. zhangyh@zzu.edu.cn
Received:2007-01-14 Accepted:2007-01-27
Abstract
AIM: To extract mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) simultaneously from the same tissues and evaluate its efficiency by comparative analysis.
METHODS: Both mtDNA and nDNA were extracted simultaneously from the same tissues using agent kit. Agarose gel electrophoresis and polymerase chain reaction were used to identify the obtained mtDNA and nDNA.
RESULTS: mtDNA and nDNA were successfully extracted from the mitochondria and nucleus of the same tissue, respectively. The efficiency of this method for extracting mtDNA and nDNA simultaneously from the same tissue was similar to that of the traditional method (extracting mtDNA and nDNA from different tissues), and there was a close relationship between these two methods ......
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