析因实验法建立规范化小鼠暴发性肝衰竭模型
D-氨基半乳糖;暴发性肝衰竭;模型;析因实验,张磐,范学工,章保新,钟白云,通讯作者:,Optimizationofmousemodeloffulminanthepaticfailurebyfactorialexperiment,PanZha
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张磐, 范学工, 中南大学湘雅医院传染科 湖南省长沙市 410008
章保新, 湖南省武警总队医院传染科 湖南省长沙市 410006
钟白云, 中南大学湘雅医院检验科 湖南省长沙市 410008
通讯作者: 范学工, 410008, 湖南省长沙市, 中南大学湘雅医院传染科. xgfan@hotmail.com
电话: 0731-4327392
收稿日期: 2007-01-14 接受日期: 2007-01-31
Optimization of mouse model of fulminant hepatic failure by factorial experiment
Pan Zhang, Xue-Gong Fan, Bao-Xin Zhang, Bai-Yun Zhong
Pan Zhang, Xue-Gong Fan,Department of Infectious Disease, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
Bao-Xin Zhang,Department of Infectious Disease, Hunan Provincial Corps Hospital, Chinese Armed Police Forces, Changsha 410006, Hunan Province, China
Bai-Yun Zhong,Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
Correspondence to:Xue-Gong Fan, Department of Infectious Disease, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. xgfan@hotmail.com
Received:2007-01-14 Accepted:2007-01-31
Abstract
AIM: To establish a stable and effective mouse model of fulminant hepatic failure (FHF) induced by D-galactosamine (D-GalN) and lipopolysaccharide (LPS).
METHODS: Two factors, attacking dosages of D-GalN and LP, influencing the success of modeling were chosen by factorial experiment. Three dosages of two factors were injected into the peritoneal cavity of mice and the death rate within 24 hours was calculated. Liver function and liver histopathological changes were also detected.
RESULTS: Two administration projects were chosen: 600 mg/kg D-GalN combined with 0.5 mg/kg LPS, or 800 mg/kg D-GalN combined with 0.04 mg/kg LPS. The death rates were affected by D-GalN and LPS in dose-dependent manner (F = 36.878, P = 0.000; F = 32.386, P = 0.000). There are interaction between D-GalN and LPS (F = 5.226, P = 0.005), and the combinatory administration of D-GalN and LPS increased the death rates of mice obviously ......
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