白花蛇舌草总黄酮抑制人肝癌细胞的靶基因调控
白花蛇舌草;总黄酮;人基因芯片;人肝癌细胞SMMC-7721;抑制作用,张硕,周三,岳旺,宋衍芹,倪同尚,张硕,通讯作者:,Targetgeneregulationinvolvedintheinhibitoryeffectoffavonoi
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张硕, 周三, 岳旺, 青岛大学医学院药学系 山东省青岛市 266021
宋衍芹, 青岛市市立医院口腔科 山东省青岛市 266071
倪同尚, 青岛大学医学院解剖教研室 山东省青岛市 266021
张硕, 2005年成都中医药大学中药学博士, 主要从事天然药物药理研究和毒理评价.
通讯作者: 岳旺, 266021, 山东省青岛市登州路38号, 青岛大学医学院药学系. dr_zhangshuo@sina.com
电话: 0532-82991888
收稿日期: 2006-12-28 接受日期: 2007-01-23
Target gene regulation involved in the inhibitory effect of favonoids from Hedyotis diffusa willd. on human hepatoma cell line SMMC-7721
Shuo Zhang, Yan-Qin Song, San Zhou, Tong-Shang Ni, Wang Yue
Shuo Zhang, San Zhou, Wang Yue,Department of Pharmacy, Medical College of Qingdao University, Qingdao 266021, Shandong Province, China
Yan-Qin Song,Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266071, Shandong Province, China
Tong-Shang Ni,Department of Anatomy, Medical College of Qingdao University, Qingdao 266021, Shandong Province, China
Correspondence to:Wang Yue, Department of Pharmacology, Medical College of Qingdao University, 38 Dengzhou Road, Qingdao 266021, Shandong Province, China. dr_zhangshuo@sina.com
Received:2006-12-28 Accepted:2007-01-23
Abstract
AIM: To investigate the target gene regulation during the inhibition of human hepatoma cell line SMMC-7721 induced by favonoids from Hedyotis diffusa willd. (FHD).
METHODS: The total RNA was extracted from normal human hepatic cells, SMMC-7721 cells and FHD-treated SMMC-7721 cells, and synthesized into double strand cDNA templates. Transcription of cRNA probe with biotin labeling was performed, and then the obtained cDNA was hybridized with human HO4 gene profile. Cy3 dye was detected with Scan array 5000, and the image information was converted into numeric data. Clusting analysis was performed with IMAGNE Ⅳ software.
RESULTS: Twenty target genes were found to be involved in the FHD-induced inhibition of human hepatoma cells. After FHD treatment, these genes were either up-regulated or down-regulated ......
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