受体骨髓间质干细胞在大鼠原位肝移植中对免疫排斥的影响
骨髓间质干细胞;原位肝移植术;免疫排斥,谭浩翔,高毅,张会迎,王琼,谭浩翔,通讯作者:,Effectonimmunologicalrejectionbyrecipient-derivedbonemarrowmesenchymalstemc
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谭浩翔, 高毅, 张会迎, 王琼, 南方医科大学珠江医院普外科 广东省广州市 510280
谭浩翔, 医学博士, 主要从事肝移植及相关的基础研究.
广东省科技计划项目, No. 2002A3020206
通讯作者: 高毅, 510280, 广东省广州市, 南方医科大学珠江医院普外科. gaoyi6146@163.com
电话: 020-61643210 传真: 020-61643291
收稿日期: 2007-01-23 接受日期: 2007-02-08
Effect on immunological rejection by recipient-derived bone marrow mesenchymal stem cells after orthotopic liver transplantation
Hao-Xiang Tan, Yi Gao, Hui-Ying Zhang, Qiong Wang
Hao-Xiang Tan, Yi Gao, Hui-Ying Zhang, Qiong Wang,Department of General Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China
Supported bythe Science and Technology Project of Guangdong Province, No. 2002A3020206
Correspondence to:Yi Gao, Department of General Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China. gaoyi6146@163.com
Received:2007-01-23 Accepted:2007-02-08
Abstract
AIM: To investigate the distribution and effect of recipient-derived bone marrow mesenchymal stem cells (BM-MSCs) after orthotopic liver transplantation.
METHODS: The model of orthotopic liver transplantation was established using two-cuff method. Wistar rats were used as donors while Sprague Dawley rats were selected as recipients. The BM-MSCs were isolated by the combination of gradient density centrifugation and adhesion separation. The collected BM-MSCs were labeled with 5, 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) and then infused into livers via portal vein after the operation. The distributions of BM-MSCs in liver, spleen, lung, and kidney were detected by fluorescence microscope 1, 2 weeks and 1 month after the operation. Hepatic functions and immunological rejection in liver tissues were also detected.
RESULTS: BM-MSCs collected by gradient density centrifugation grew in an adhesion mode on the wall and had a ball-like appearance ......
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