腹腔液中CD44和整合素β1表达与胃癌腹膜转移的关系
胃肿瘤;腹膜种植性转移;CD44;整合素β1,赵东晖,徐惠绵,孙秀菊,张世浦,通讯作者:,ExpressionofCD44andintegrinβ1inperitoneallavagefluidandtheircorrelation
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赵东晖, 徐惠绵, 中国医科大学附属第一医院肿瘤外科 辽宁省沈阳市 110001
孙秀菊, 中国医科大学医学遗传学教研室 辽宁省沈阳市 110001
张世浦, 沈阳医学院沈洲医院普外科 辽宁省沈阳市 110002
辽宁省科学技术计划资助项目, No. 2005225005
沈阳医学院科学研究基金资助项目, No. 20061004
通讯作者: 徐惠绵, 110001, 辽宁省沈阳市和平区南京北街155号, 中国医科大学附属第一医院肿瘤外科. huimianxu@163.com
电话: 024-83283555 传真: 024-22703578
收稿日期: 2007-01-15 接受日期: 2007-02-08
Expression of CD44 and integrin β1 in peritoneal lavage fluid and their correlations with the peritoneal metastasis of gastric cancer
Dong-Hui Zhao, Hui-Mian Xu, Xiu-Ju Sun, Shi-Pu Zhang
Dong-Hui Zhao, Hui-Mian Xu,Department of Oncological Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Xiu-Ju Sun,Department of Medical Genetics, China Medical University, Shenyang 110001, Liaoning Province, China
Shi-Pu Zhang,Department of General Surgery, Shenzhou Hospital of Shenyang Medical College, Shenyang 110002, Liaoning Province, China
Supported bythe Science and Technology Project of Liaoning Province, No. 2005225005, and the Research Foundation of Shenyang Medical College, No. 20061004
Correspondence to:Hui-Mian Xu, Department of Oncological Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. huimianxu@163.com
Received:2007-01-15 Accepted:2007-02-08
Abstract
AIM: To investigate the expression of CD44 and integrin β1 mRNA in peritoneal lavage fluid and their correlations with the peritoneal metastasis of gastric cancer.
METHODS: The samples of peritoneal lavage fluid were collected during operation from 50 patients with gastric cancer. The expression of CD44 and integrin β1 was detected at mRNA level by reverse transcription-polymerase chain reaction (RT-PCR), and the examination of peritoneal lavage cytology (PLC) was also performed. All the cases were divided into different groups according to the invasion depth of foci, serosal types, results of PLC, peritoneal metastasis and TNM stages ......
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