VEGF反义寡核苷酸对胆囊癌细胞VEGF, Flt-1及KDR mRNA表达和VEGF蛋白分泌的影响
胆囊癌细胞GBC-SD;血管内皮生长因子;fms样酪氨酸激酶1;含激酶插入区受体;反义寡核苷酸;半定量RT-PCR;酶链免疫吸附试验,VEGF反义寡核苷酸对胆囊癌细胞VEGF,Flt-1及KDRmRNA表达和VEGF蛋白分泌的影响,李海军,庞作良,毛拉艾沙·买买提,李海军,通讯作者:,Eff
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李海军, 庞作良, 毛拉艾沙·买买提, 新疆医科大学附属肿瘤医院肝胆胰外科 新疆省乌鲁木齐市 830011
李海军, 2005年浙江大学医学院医学博士, 副教授, 副主任医师, 主要从事肝胆胰肿瘤的基础与临床研究.
通讯作者: 庞作良, 830011, 新疆乌鲁木齐市北京南路30号, 新疆医科大学附属肿瘤医院肝胆胰外科. lhjun3408@163.com
电话: 0991-7819111 传真: 0991-7819096
收稿日期: 2006-12-26 接受日期: 2007-01-27
Effects of vascular endothelial growth factor antisense oligodeoxynucleotide on the mRNA expression of vascular endothelial growth factor, fms-like tyrosine kinase-1 and kinase insert domain-containing receptor and vascular endothelial growth factor protein excretion of gallbladder carcinoma GBC-SD cells in vitro
Hai-Jun Li, Zuo-Liang Pang, Mao-La-Ai-Sha Mai-Mai-Ti
Hai-Jun Li, Zuo-Liang Pang, Mao-La-Ai-Sha Mai-Mai-Ti,Department of Hepatobiliary and Pancreatic Surgery, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
Correspondence to:Zuo-Liang Pang, Department of Hepatobiliary and Pancreatic Surgery, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China. lhjun3408@163.com
Received:2006-12-26 Accepted:2007-01-27
Abstract
AIM: To investigate the effect of oligofectamine-mediated vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotide (ASODN) transfection on the mRNA expression of VEGF, fms-like tyrosine kinase-1 (Flt-1) and kinase insert domain-containing receptor (KDR) as well as VEGF protein excretion of gallbladder carcinoma GBC-SD cells in vitro.
METHODS: Gallbladder carcinoma GBC-SD cells were transfected with VEGF ASODN and scrambled oligodeoxynucleotide (SODN) by Oligofectamine mediation. The mRNA expression of VEGF, Flt-1 and KDR in GBC-SD cells of each group were detected by semi-quantitive reverse transcription-polymerase chain reaction (RT-PCR) and the excretion of VEGF protein was measured by enzyme-linked immunosorbent assay (ELISA) ......
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