肠道细菌CGC定植及其对局部细胞活性的影响
肠易激综合征;肠道细菌潜生体;回盲部;线粒体,刘俊康,廖玉芳,胡峻晨,吴小兰,徐启旺,刘俊康,通讯作者:,Colonizationofbacterialcrypticgrowthcellsanditsinfluenceonthelocalcellu
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刘俊康, 廖玉芳, 胡峻晨, 吴小兰, 徐启旺, 中国人民解放军第三军医大学医学检验系生物波研究室 重庆市 400038
刘俊康, 医学博士, 副教授, 主要从事临床检验及生物复杂性等方面的研究.
重庆市科委自然科学基金计划资助项目, No. 2006BB5068
通讯作者: 徐启旺, 400038, 重庆市, 中国人民解放军第三军医大学医学检验系生物波研究室. biowave2000@163.com
收稿日期: 2006-12-04 接受日期: 2007-02-08
Colonization of bacterial cryptic growth cells and its influence on the local cellular activity
Jun-Kang Liu, Yu-Fang Liao, Jun-Chen Hu, Xiao-Lan Wu, Qi-Wang Xu
Jun-Kang Liu, Yu-Fang Liao, Jun-Chen Hu, Xiao-Lan Wu, Qi-Wang Xu,Research Center of Bio-wave, Department of Laboratory Medicine, the Third Military Medical University, Chongqing 400038, China
Supported bythe Natural Science Planning Foundation of Chongqing Science and Technology Committee, No. 2006BB5068
Correspondence to:Qi-Wang Xu, Research Center of Bio-wave, Department of Laboratory Medicine, the Third Military Medical University, Chongqing 400038, China. biowave2000@163.com
Received: 2006-12-04 Accepted:2007-02-08
Abstract
AIM: To explore the predominant location and the influence of bacterial cryptic growth cell (CGC) colonization on the activity of local enterocytes.
METHODS: The bacterial CGC was induced by the antibiotics Cefuroxime, and the colonized status was detected by the techniques of local colonization in vivo. After the establishment of irritable bowel syndrome (IBS) model, the energy charge (EC) of adenosine triphosphate (ATP) and the ratio of ATP to the total amount of adenylic acid pool in colonic epithelial cells at the ileocecum with better colonization were determined by high performance liquid chromatography (HPLC) ......
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