转铁蛋白阿霉素脂质体的制备及其对人肝癌细胞系的杀伤活性
转铁蛋白;脂质体;靶向作用;肝细胞癌;阿霉素,秦历杰,刘占举,通讯作者:,PreparationofadriamycinliposomecoupledwithHTf(Fe)2anditsanti-tumoractivityonhuman
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秦历杰, 河南省人民医院急诊科 河南省郑州市 450003
刘占举, 郑州大学第二附属医院消化内科 河南省郑州市 450014
通讯作者: 刘占举, 450014, 河南省郑州市经八路2号, 郑州大学第二附属医院消化内科. zhanjuliu@yahoo.com
电话: 0371-63939084
收稿日期: 2006-11-03 接受日期: 2007-03-06
Preparation of adriamycin liposome coupled with HTf(Fe)2 and its anti-tumor activity on human hepatoma cell line SMMC-7721
Li-Jie Qin, Zhan-Ju Liu
Li-Jie Qin,Department of Emergency Management, He'nan Provincial People’s Hospital, Zhengzhou 450003, He'nan Province, China
Zhan-Ju Liu,Department of Gastroenterology, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, He'nan Province, China
Correspondence to:Zhan-Ju Liu, Department of Gastroenterology, the Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou 450014, He'nan Province, China. zhanjuliu@yahoo.com
Received: 2006-11-03 Accepted: 2007-03-06
Abstract
AIM: To modify the adriamycin (ADM) liposome with transferrin HTf(Fe)2 according to the difference of receptor or antigen expression between tumor cells and normal cells, and study its efficacy of anti-tumor activities on human hepatoma cell line SMMC-7721.
METHODS: Cross-linking reagent (SPDP) reacting with human transferrin HTf(Fe)2 was utilized, and liposomes coupled with transferrin [named as HTf(Fe)2-ADM-liposome] were prepared. Then human hepatoma cell line SMMC-7721 was treated with different concentrations of HTf(Fe)2-ADM-liposome and MTT assay was used to the killing effect on SMMC-7721 cells.
RESULTS: The success rate of HTf(Fe)2 coupling with liposomes was 73.5%. Electron microscopy showed no significant difference in the diameters between HTf(Fe)2-ADM-liposome and ADM-liposome (56 ± 38 nm vs 54 ± 30 nm, P < 0.05). Modification and coupling didn’t affect the activity of HTf(Fe)2 ......
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