幽门螺杆菌NCTC 11637 cagT基因的克隆及序列分析
幽门螺杆菌;cagT;克隆;测序,幽门螺杆菌NCTC11637cagT基因的克隆及序列分析,崔蕾蕾,邵世和,通讯作者:,CloningandsequenceanalysisofHelicobacterpyloriNCTC11637cagTgene,Lei-LeiCui,Shi-HeShao
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崔蕾蕾, 邵世和, 江苏大学医学技术学院病原学及病原学检验系 江苏省镇江市 212013
江苏省科技厅项目: No. BS2004021
江苏大学高级人才项目: No. JDG2004008
通讯作者: 邵世和, 212013, 江苏省镇江市学府路301号, 江苏大学医学技术学院病原学及病原学检验系.
shaoshihe2006@163.com
电话: 0511-5038375-326 传真: 0511-5038449
收稿日期: 2007-01-17 接受日期: 2007-02-12
Cloning and sequence analysis of Helicobacter pylori NCTC 11637 cagT gene
Lei-Lei Cui, Shi-He Shao
Lei-Lei Cui, Shi-He Shao,Department of Etiology and Etiology Inspection, School of Medical Technology, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China
Supported bythe Science and Technology Office Program of Jiangsu Province, No. BS2004021 and the High-Grade Talented Individuals Program of Jiangsu University, No. JDG2004008
Correspondence to:Shi-He Shao, Department of Etiology and Etiology Inspection, School of Medical Technology, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China. shaoshihe2006@163.com
Received:2007-01-17 Accepted:2007-02-12
Abstract
AIM: To clone and analyze H pylori cagT gene.
METHODS: H pylori cagT gene was amplified from the genome DNA by polymerase chain reaction (PCR). The PCR product was inserted into pGEM-T vector and then transformed into E.coli DH5α. The positive recombinant clone was analyzed by digestion of restriction endonuclease. Next, the cagT gene fragment was inserted directionally into vector pQE30 to construct recombinant clone of cagT, which was sequenced finally.
RESULTS: A cagT gene consisting of 843 base pairs, which encoded a product of 280 amino acids, was obtained using PCR method and was cloned into pGEM-T vector successfully. Its GenBank accession number was EF114758. Sequence analysis showed that cagT gene had shared 97%-99% homology with other strains in GenBank.
CONCLUSION: The correct cagT gene is successfully cloned, which established a basis for further investigation of its biological function ......
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