丙酮酸乙酯对急性坏死性胰腺炎大鼠肺损伤的保护作用
高迁移率族蛋白-1;急性坏死性胰腺炎;急性肺损伤;丙酮酸乙酯;酶联免疫吸附试验;逆转录-聚合酶联反应,朱峰,葛春林,郭仁宣,朱峰,通讯作者:,Protectiveeffectsofethylpyruvateagainstacutelungin
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朱峰, 葛春林, 郭仁宣, 中国医科大学附属第一医院普外二科 辽宁省沈阳市 110001
朱峰, 医学博士, 主要从事重症急性胰腺炎诊治方面的研究.
通讯作者: 郭仁宣, 110001, 辽宁省沈阳市, 中国医科大学附属第一医院普外二科. zhuzhufeng-7@163.com
电话: 024-83283330
收稿日期: 2006-12-06 接受日期: 2007-02-08
Protective effects of ethyl pyruvate against acute lung injury induced by acute necrotizing pancreatitis in rats
Feng Zhu, Chun-Lin Ge, Ren-Xuan Guo
Feng Zhu, Chun-Lin Ge, Ren-Xuan Guo,Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to:Dr. Ren-Xuan Guo, Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. zhuzhufeng-7@163.com
Received:2006-12-06 Accepted:2007-02-08
Abstract
AIM: To investigate the effects of ethyl pyruvate (EP) on the levels of tumor necrosis factor-α (TNF-α), interleukin-1b (IL-1b) in serum and mRNA expression of high mobility group box 1 (HMGB1) in lung tissues, and explore the mechanisms of ethyl pyruvate in protecting the lungs against acute injury induced by acute necrotizing pancreatitis (ANP).
METHODS: ANP model was induced by retrograde injection of 50 g/L sodium taurocholate into pancreatic duct. Animals were divided randomly into 3 groups: control group, ANP group, and EP treatment group. EP solution was administered intravenously every 6 hours (40 mg/kg once). The serum levels of TNF-α and IL-1b were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of HMGB1 in lung tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR). The partial pressure of blood oxygen (PaO2) was measured, and histological examination of the lungs was also performed.
RESULTS: The serum levels of TNF-α and IL-6 were rapidly increased after ANP model was induced, and reached peak at the 6th hour, but they were decreased at the 12th hour ......
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