氧化苦参碱对人结肠癌细胞P21, P27, Cyclin E1及CDK2表达的影响
结肠癌;氧化苦参碱;细胞周期;流式细胞术;逆转录-聚合酶链反应;免疫印迹,氧化苦参碱对人结肠癌细胞P21,P27,CyclinE1及CDK2表达的影响,陆丽华,冉志华,陆丽华,通讯作者:,EffectsofoxymatrineontheexpressionofP21,P27,CyclinE1
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陆丽华, 冉志华, 上海交通大学医学院附属仁济医院、上海市消化疾病研究所 上海市 200001
陆丽华, 医学硕士, 主要从事消化道肿瘤的基础研究.
通讯作者: 冉志华, 200001, 上海市, 上海交通大学医学院附属仁济医院消化科, 上海市消化疾病研究所. z-ran@online.sh.cn
电话: 021-58752345 传真: 021-63266027
收稿日期: 2007-01-29 接受日期: 2007-03-26
Effects of oxymatrine on the expression of P21, P27, Cyclin E1 and CDK2 in human colon cancer cell line SW1116
Li-Hua Lu, Zhi-Hua Ran
Li-Hua Lu, Zhi-Hua Ran,Renji Hospital, School of Medicine, Shanghai Jiao Tong University; Shanghai Institute of Digestive Disease, Shanghai 200001, China
Correspondence to:Zhi-Hua Ran, Renji Hospital, School of Medicine, Shanghai Jiao Tong University; Shanghai Institute of Digestive Disease, Shanghai 200001, China. z-ran@online.sh.cn
Received:2007-01-29 Accepted:2007-03-26
Abstract
AIM: To observe the effect of oxymatrine (OM) on the expression of P21, P27, Cyclin E1 and CDK2 in human colon cancer cell line SW1116, investigate the pathways of OM in inhibiting tumor cell proliferation, and explore its anti-tumor mechanism.
METHODS: Human colon cancer cell line SW1116 was cultured and then treated with different concentrations (2, 3, 4 g/L) of OM, respectively, for 24 and 48 hours. Flow cytometry was used to detect the cycle changes of SW1116 cells. Reverse transcription polymerase chain reaction and Western blot were performed to measure the expression of P21, P27, Cyclin E1 and CDK2 at mRNA and protein level.
RESULTS: In comparison with those in control group, the percentages of G1/G0-phase cells were significantly increased after treatment with 2, 3, 4 g/L OM (24 h: 67.5% ± 0.1%, 69.5% ± 1.4%, 71.0% ± 1.0% vs 58.6% ± 0.4%, P < 0.05; 48 h: 68.5% ± 0.3%, 71.9% ± 0.9%, 78.0% ± 0.4% vs 58.8% ± 0.1%, P < 0.05); the mRNA and protein expression of Cyclin E1 was down-regulated while those of P21 and P27 were up-regulated (P < 0.05); the mRNA expression of CDK2 was lowered, but the protein expression of CDK2 had no significant changes (P > 0 ......
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