不同转移潜能肝癌细胞株的差异蛋白质组的二维液相色谱分析
蛋白质组;肝细胞癌;肿瘤转移;二维液相色谱分离;MALDI-TOF质谱,彭艳,蒋平,焦炳华,张霞,王葵,彭艳,倪健,彭艳,通讯作者:,Proteomeanalysisofhepatocellularcarcinomacell
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彭艳, 蒋平, 焦炳华, 第二军医大学基础医学部生物化学与分子生物学教研室 上海市 200433
张霞, 上海交通大学药学院 上海市 200240
王葵, 第二军医大学东方肝胆外科医院 上海市 200438
彭艳, 倪健, 上海晨健抗体组药物有限公司 201203
彭艳, 第二军医大学生物化学与分子生物学博士, 上海张江高科技园区企业博士后, 主要从事肿瘤相关蛋白功能研究.
上海市科学技术委员会资助课题, No. 054319938
通讯作者: 倪健, 201203, 上海市李冰路306号, 上海晨健抗体组药物有限公司. jiannihome@yahoo.com
电话: 021-51388210 传真: 021-51388211
收稿日期: 2007-01-31 接受日期: 2007-02-22
Proteome analysis of hepatocellular carcinoma cell lines with different metastasis potentials by two-dimensional liquid chromatography
Yan Peng, Xia Zhang, Kui Wang, Ping Jiang, Bing-Hua Jiao, Jian Ni
Yan Peng, Ping Jiang, Bing-Hua Jiao,Department of Biochemistry and Molecular Biology, the Second Military Medical University, Shanghai 200433, China
Xia Zhang,School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China
Kui Wang,Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200438, China
Yan Peng, Jian Ni,Shanghai Human Antibodomics Inc, Shanghai 201203, China
Supported bythe Science and Technology Commission Foundation of Shanghai, No. 054319938
Correspondence to:Jian Ni, Shanghai Human Antibodomics Inc, 306 Libing Road, Shanghai 201203,China. jiannihome@yahoo.com
Received:2007-01-31 Accepted:2007-02-22
Abstract
AIM: To analyze the proteome of hepatocellular carcinoma cell lines, MHCC97-H (high metastasis) and MHCC97-L (low metastasis), with different metastasis potentials by two-dimentional liquid phase chromatographic fractionation followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS).
METHODS: Cell lysates of MHCC97-H and MHCC97-L were fractionated according to PI using chromatofocusing with analytical columns in the first dimension, followed by separation of the proteins in each PI fraction using nonporous reversed-phase high performance liquid chromatography (HPLC) ......
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