AG490联合健择对人胰腺癌细胞生长的影响
胰腺肿瘤;信号转导与转录激活因子3;AG490;增殖;凋亡;MTT法;流式细胞术;免疫印迹;逆转录-聚合酶链反应,陈钢,裘正军,黄陈,陈钢,通讯作者:,EffectofAG490combinedwithgemcitabineonthegrowthofh
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陈钢, 裘正军, 黄陈, 上海交通大学附属第一人民医院普外科 上海市 200080
陈钢, 2001年毕业于南京医科大学, 2004年考入上海交通大学医学院硕士研究生, 主要从事胰腺癌的基础和临床研究.
通讯作者: 裘正军, 200080, 上海市, 上海交通大学附属第一人民医院普外科. qiuwryb@online.sh.cn
电话: 021-63240090-3092
收稿日期: 2007-03-06 接受日期: 2007-03-23
Effect of AG490 combined with gemcitabine on the growth of human pancreatic cancer cells
Gang Chen, Zheng-Jun Qiu, Chen Huang
Gang Chen, Zheng-Jun Qiu, Chen Huang, Department of General Surgery, the Affiliated First People's Hospital of Shanghai Jiao Tong University, Shanghai 200080, China
Correspondence to: Zheng-Jun Qiu, Department of General Surgery, the Affiliated First People's Hospital, Shanghai Jiao Tong University, Shanghai, China. qiuwryb@online.sh.cn
Received: 2007-03-06 Accepted: 2007-03-23
Abstract
AIM: To investigate the effect of Janus kinase (JAK) specific inhibitor AG490 combined with gemcitabine on the proliferation of human pancreatic cancer cell line SW1990 and STAT3 signal transduction pathway as well as their mechanisms.
METHODS: Human pancreatic cancer cell line SW1990 was divided into control group, AG490-treated group, gemcitabine-treated group and AG490 + gemcitabine-treated group. After 48 hours, the proliferation of SW1990 cells was detected by MTT assay. Flow cytometry was used to examine cell apoptosis. The expression of STAT3, Cyclin D1, Bcl-xL and Bax were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively.
RESULTS: The proliferation of SW1990 cells was significantly lower in AG490 or gemcitabine group than that in control group (2.20 ± 0.25, 2.30 ± 0.220 vs 3.78 ± 0.42, P < 0.05), but the apoptosis rate was markedly higher (35.40% ± 3.08%, 34.64% ± 1.38% vs 16.49% ± 1.45%, P < 0.05). Moreover, the proliferation (1.49 ± 0.15) and apoptosis (43.80% ± 1.57%) had notable difference between AG490+gemcitabine group and AG490 or gemcitabine group ......
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