肾母细胞瘤过度表达基因对大鼠肝星状细胞生物学行为的影响
肾母细胞瘤过度表达基因;小干扰RNA;肝星状细胞;半定量RT-PCR,徐冬,林菊生,任精华,陈琼,姚津剑,何星星,徐冬,通讯作者:,Effectofnephroblastomaoverexpressedgeneonbiologicalbeh
 |
| 第6页 |
 |
| 第1页 |
参见附件(976KB，7页)。
徐冬, 林菊生, 任精华, 陈琼, 姚津剑, 何星星, 华中科技大学同济医学院附属同济医院肝病研究所 湖北省武汉市 430030
徐冬, 华中科技大学同济医学院硕士生, 主要从事肝胆疾病的研究.
通讯作者: 林菊生, 430030, 湖北省武汉市, 华中科技大学同济医学院附属同济医院肝病研究所. jslin@tjh.tjmu.edu.cn
电话: 027-83662578 传真: 027-83662578
收稿日期: 2007-01-30 接受日期: 2007-02-13
Effect of nephroblastoma overexpressed gene on biological behaviors of rat hepatic stellate cells
Dong Xu, Ju-Sheng Lin, Jing-Hua Ren, Qiong Chen, Jin-Jian Yao, Xing-Xing He
Dong Xu, Ju-Sheng Lin, Jing-Hua Ren, Qiong Chen, Jin-Jian Yao, Xing-Xing He, Institute of Liver Diseases, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Correspondence to: Ju-Sheng Lin, Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. jslin@tjh.tjmu.edu.cn
Received: 2007-01-30 Accepted: 2007-02-13
Abstract
AIM: To construct recombinant plasmids that can express small interfering RNA (siRNA) targeting nephroblastoma overexpressed gene (NOV) and investigate its effect on the biological behaviors of hepatic stellate cells (HSCs).
METHODS: Hairpin siRNA templates targeting NOV gene were synthesized and cloned into plasmid vector psiRNA-H1neo. Three vector-derived siRNAs (denoted psiRNA1, 2 and 3) and one mocking pconsiRNA (as control) were constructed. The recombinant NOV siRNA plasmids were constructed and identified using restrictive enzyme analysis and DNA sequencing, and then transfected into HSCs by lipofectamine. HSCs were divided into group psiRNA1, psiRNA2, psiRNA3 and pconsiRNA, transfected with the corresponding recombinant plasmids. Blank group consisted of HSCs contaning no plasmids. The expression of NOV and α-smooth muscle actin (α-SMA) were detected by semi-quantitative reverser transcription-polymerase chain reaction (RT-PCR) and Western blot ......
您现在查看是摘要介绍页，详见PDF附件(976KB，7页)。