人末梢血γδT细胞对消化系统肿瘤细胞的杀伤作用
γδT细胞;细胞毒活性;消化系肿瘤;过继性免疫治疗,陈复兴,刘军权,王涛,张娟,张颂,陈桂林,冯霞,陈复兴,通讯作者:,CytotoxiceffectsofhumanperipheralbloodγδTcellsonthetumorcellsof
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陈复兴, 刘军权, 王涛, 张娟, 张颂, 陈桂林, 徐州解放军第97医院实验科 江苏省徐州市 221004
冯霞, 徐州医学院附属医院实验科 江苏省徐州市221002
陈复兴, 主任技师, 主要从事肿瘤生物治疗研究.
南京军区医学科学技术研究“十一五”计划课题资助项目, No. 06MA45
通讯作者: 陈复兴, 221004, 江苏省徐州市铜山路226号, 中国人民解放军第97医院实验科. chengFuxin123@126.com
电话: 0516-83349134
收稿日期: 2006-11-30 接受日期: 2007-03-31
Cytotoxic effects of human peripheral blood γδT cells on the tumor cells of digestive systemin vitro
Fu-Xing Chen, Jun-Quan Liu, Xia Feng, Tao Wang, Juan Zhang, Song Zhang, Gui-Lin Chen
Fu-Xing Chen, Jun-Quan Liu, Tao Wang, Juan Zhang, Song Zhang, Gui-Lin Chen, Department of Central Laboratory, the 97th Hospital of Chinese PLA, Xuzhou 221004, Jiangsu Province, China
Xia Feng, Department of Laboratory, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
Supported by the Medical Science and Technology Foundation of Nanjing Military Region during the 11th Five-Year Plan Program, No. 06MA45
Correspondence to: Fu-Xing Chen, Department of Central Laboratory, the 97th Hospital of Chinese PLA, 226 Tongshan Road, Xuzhou 221004, Jiangsu Province, China. chengfuxin123@126.com
Received: 2006-11-30 Accepted: 2007-03-31
Abstract
AIM: To investigate the cytotoxic effects of human peripheral blood γδT cells on the tumor cell lines of digestive system.
METHODS: The γδT cells in peripheral blood of healthy volunteers were expanded using interleukin-2 (IL-2) and isopentenyl pyrophodphate (IPP) in PRMI 1640 medium. After 10 days, the purity of γδT cells was analyzed by flow cytometry. The cytotoxic activity of the purified γδT cells against human gastric cancer cells, pancreatic carcinoma cells and hepatocellular carcinoma cells were tested in different effector-to-target ratios.
RESULTS: After peripheral blood mononuclear cells were cultured for 10 days, the γδT cells were expanded rapidly from 4 ......
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