氧化苦参碱对人胃癌细胞杀伤作用的机制
氧化苦参碱;胃癌;端粒酶;聚合酶链反应;酶联免疫吸附测定;四唑蓝比色法;流式细胞仪,于晓峰,邹健,冉志华,通讯作者:,KillingeffectofoxymatrineonhumangastriccancercelllineMKN45anditsmech
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于晓峰, 邹健, 上海市华东医院消化科 上海市 200040
冉志华, 上海市仁济医院消化科 上海市 200001
通讯作者: 于晓峰, 200040, 上海市延安西路221号, 上海市华东医院消化科. yuxfeng@smmail.cn
电话: 021-62483180
收稿日期: 2006-12-07 接受日期: 2007-01-10
Killing effect of oxymatrine on human gastric cancer cell line MKN45 and its mechanism
Xiao-Feng Yu, Jian Zou, Zhi-Hua Ran
Xiao-Feng Yu, Jian Zou,Department of Digestology, Shanghai Huadong Hospital, Shanghai 200040, China
Zhi-Hua Ran, Department of Digestology, Shanghai Renji Hospital, Shanghai 200001, China
Correspondence to:Xiao-Feng Yu, Department of Digestology, Shanghai Huadong Hospital, 221 Yan’an West Road, Shanghai 200040, China. yuxfeng@smmail.cn
Received:2006-12-07 Accepted:2007-01-10
Abstract
AIM: To explore the killing effects of oxymatrine (OM) on human gastric cancer cell line MKN45 and its anti-neoplastic mechanism.
METHODS: Human gastric cancer cell line MKN45 was cultured and then treated with 0.5, 1, 2, 4 and 6 g/L OM. Methylthiazolyl tetrazolium analysis (MTT) was used to observe the killing effect of OM on MKN45 cells. Cell cycle distribution was measured by flow cytometry. The activity of telomerase was detected by polymerase chain reaction (PCR)-enzyme linked immunosorbent assay (ELISA). Reverse transcription (RT)-PCR was employed to examine the expression of hTERT, c-myc, p53 and mad1 genes in MKN45 cells.
RESULTS: OM exhibited dose-dependent killing effects on MKN45 cells and its IC50 was 2.78 g/L. After administration for 48 hours, OM induced an increase of G1/G0-phase cells (62.2% ± 1.3% vs 56.7% ± 4.0%, P < 0.05) and decrease of G2/M-phase cells (5.4% ± 1.1% vs 10.0% ± 2.8%, P < 0.05) at a dose of 2 g/L, and a decrease of S-phase cells (30.5% ± 1.3% vs 33.4% ± 1.2%, P < 0.05) at a dose of 4 g/L. OM inhibited the activity of telomerase in MKN45 cells in a dose-dependent manner. The expression of hTERT gene in MKN45 cells was decreased, but the expression of p53 and mad1 genes were increased ......
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