扶正利湿解毒法体内外逆转肝纤维化
肝纤维化;逆转作用;复方;Masson染色;细胞凋亡,何金洋,邓文娣,周映云,符路娣,通讯作者:,Invivoandinvitrostudyonliverfibrosisreversioneffectbytherapyofstrengtheninghealthy
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何金洋, 邓文娣, 周映云, 广州中医药大学热带医学研究所 广东省广州市 510405
符路娣, 广州中医药大学实验动物中心 广东省广州市 510405
何金洋, 博士, 助理研究员, 主要从事乙型病毒性肝炎、艾滋病等病毒性疾病研究.
广州中医药大学科研创新基金, No.k0050044
通讯作者: 何金洋, 510405, 广东省广州市机场路12号, 广州中医药大学热带医学研究所. sunny12345678_89@yahoo.com.cn
电话: 020-31774402
收稿日期: 2007-03-06 接受日期: 2007-03-23
In vivo and in vitro study on liver fibrosis reversion effect by therapy of strengthening healthy qi combined with draining dampness and relieving toxin
Jin-Yang He, Lu-Di Fu, Wen-Di Deng, Ying-Yun Zhou
Jin-Yang He, Wen-Di Deng, Ying-Yun Zhou, Tropical Medical Institute, Guangzhou university of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Lu-Di Fu, Laboratory Animal Center, Guangzhou university of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Correspondence to: Jin-Yang He, Tropical Medical Institute, Guangzhou University of Traditional Chinese Medicine, 12 Jichang Road, Guangzhou 510405, Guangdong Province, China. sunny12345678_89@yahoo.com.cn
Received: 2007-03-06 Accepted: 2007-03-23
Abstract
AIM: To observe the reversing effect on liver fibrosis by the therapy of strengthening healthy qi combined with draining dampness and relieving toxin (SHQDDRT).
METHODS: Rat model of liver fibrosis was induced by dimethylnitrosamine (DMN). All the rats were randomly divided into 6 groups, named group A (control, n = 10), B (model, n = 10), C (colchicine, n = 10), D (with the basic herb complex, high dose, n = 10), E (with the basic herb complex, middle dose, n = 10) and F (with the basic herb complex, low dose, n = 10). The content of liver hydroxyproline and the serum alanine aminotransferase (ALT) were tested. HE and Masson staining were conducted for pathological examination. HSC-T6 cell line was used as an in vitro model. After 24 hours of drug administration, the inhibitory effect was observed under an optical microscope. Cell proliferation was detected by MTT assay. Additionally, HSC-T6 cells were observed by electron microscopy after they were treated with the basic herb complex and control drug at the concentration of 2 g/L.
RESULTS: The content of liver hydroxyproline and the serum ALT in group D and E were significantly lower than those in group B (hydroxyproline: 213.73 ± 58.05 μg/g, 245.95 ± 67.70 μg/g vs 317.37 ± 42.06 μg/g,P < 0.01, P < 0.05; ALT: 33.56 ± 8.16 kU/L, 42.38 ± 14.99 kU/L vs 58 ± 10.32 kU/L, P < 0.01, P < 0.05). Masson staining showed that the fibrotic degrees were significantly decreased in group D and E in contrast with those in group B (2.33 ± 0.87, 2.63 ± 0.74 vs 3.70 ± 0.67, both P < 0.01), and the fiber deposition, inflammation and hemorrhage were also improved. Light endoscopy and MTT assay demonstrated that the basic herb complex inhibited the growth of HSC-T6 cells in a dose-dependent manner. Electron microscopy exhibited typical apoptosis features in HSC-T6 cells ......
何金洋, 邓文娣, 周映云, 广州中医药大学热带医学研究所 广东省广州市 510405
符路娣, 广州中医药大学实验动物中心 广东省广州市 510405
何金洋, 博士, 助理研究员, 主要从事乙型病毒性肝炎、艾滋病等病毒性疾病研究.
广州中医药大学科研创新基金, No.k0050044
通讯作者: 何金洋, 510405, 广东省广州市机场路12号, 广州中医药大学热带医学研究所. sunny12345678_89@yahoo.com.cn
电话: 020-31774402
收稿日期: 2007-03-06 接受日期: 2007-03-23
In vivo and in vitro study on liver fibrosis reversion effect by therapy of strengthening healthy qi combined with draining dampness and relieving toxin
Jin-Yang He, Lu-Di Fu, Wen-Di Deng, Ying-Yun Zhou
Jin-Yang He, Wen-Di Deng, Ying-Yun Zhou, Tropical Medical Institute, Guangzhou university of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Lu-Di Fu, Laboratory Animal Center, Guangzhou university of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
Correspondence to: Jin-Yang He, Tropical Medical Institute, Guangzhou University of Traditional Chinese Medicine, 12 Jichang Road, Guangzhou 510405, Guangdong Province, China. sunny12345678_89@yahoo.com.cn
Received: 2007-03-06 Accepted: 2007-03-23
Abstract
AIM: To observe the reversing effect on liver fibrosis by the therapy of strengthening healthy qi combined with draining dampness and relieving toxin (SHQDDRT).
METHODS: Rat model of liver fibrosis was induced by dimethylnitrosamine (DMN). All the rats were randomly divided into 6 groups, named group A (control, n = 10), B (model, n = 10), C (colchicine, n = 10), D (with the basic herb complex, high dose, n = 10), E (with the basic herb complex, middle dose, n = 10) and F (with the basic herb complex, low dose, n = 10). The content of liver hydroxyproline and the serum alanine aminotransferase (ALT) were tested. HE and Masson staining were conducted for pathological examination. HSC-T6 cell line was used as an in vitro model. After 24 hours of drug administration, the inhibitory effect was observed under an optical microscope. Cell proliferation was detected by MTT assay. Additionally, HSC-T6 cells were observed by electron microscopy after they were treated with the basic herb complex and control drug at the concentration of 2 g/L.
RESULTS: The content of liver hydroxyproline and the serum ALT in group D and E were significantly lower than those in group B (hydroxyproline: 213.73 ± 58.05 μg/g, 245.95 ± 67.70 μg/g vs 317.37 ± 42.06 μg/g,P < 0.01, P < 0.05; ALT: 33.56 ± 8.16 kU/L, 42.38 ± 14.99 kU/L vs 58 ± 10.32 kU/L, P < 0.01, P < 0.05). Masson staining showed that the fibrotic degrees were significantly decreased in group D and E in contrast with those in group B (2.33 ± 0.87, 2.63 ± 0.74 vs 3.70 ± 0.67, both P < 0.01), and the fiber deposition, inflammation and hemorrhage were also improved. Light endoscopy and MTT assay demonstrated that the basic herb complex inhibited the growth of HSC-T6 cells in a dose-dependent manner. Electron microscopy exhibited typical apoptosis features in HSC-T6 cells ......
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