IκB激酶β在非酒精性脂肪性肝炎发病机制中的作用
IκB激酶β;非酒精性脂肪性肝炎;核因子-κB,赵彩彦,王亚东,周俊英,赵彩彦,通讯作者:,RoleofthekappaBkinasebetainhibitorinthepathogenesisofnonalcoholicsteatohepati
 |
| 第3页 |
 |
| 第1页 |
参见附件(961KB，6页)。
赵彩彦, 王亚东, 周俊英, 河北医科大学第三医院感染科 河北省石家庄市 050051
赵彩彦, 2004年河北医科大学博士, 教授, 主要从事肝病的基础及临床研究.
河北省自然科学基金资助项目, No. C200500780
通讯作者: 赵彩彦, 050051, 河北省石家庄市自强路139号, 河北医科大学第三医院感染科. zhaoyy63811@vip.sohu.com
电话: 0311-87027951-2352
收稿日期: 2007-02-02 接受日期: 2007-03-23
Role of the kappa B kinase beta inhibitor in the pathogenesis of nonalcoholic steatohepatitis
Cai-Yan Zhao, Ya-Dong Wang, Jun-Ying Zhou
Cai-Yan Zhao, Ya-Dong Wang, Jun-Ying Zhou, Department of Infectious Diseases, the Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei Province, China
Supported by: the Natural Science Foundation of Hebei Province, No. C200500780
Correspondence to: Cai-Yan Zhao, Department of Infectious Diseases, the Third Hospital of Hebei Medical University, 139 Ziqiang Road, Shijiazhuang 050051, Hebei Province, China. zhaoyy63811@vip.sohu.com
Received: 2007-02-02 Accepted: 2007-03-23
Abstract
AIM: To investigate the expression and significance of the inhibitor kappa B kinase beta (IKKβ) in livers of rats with non-alcoholic steatohepatitis (NASH).
METHODS: Forty healthy male Wistar rats were randomly divided into two equal groups. Rats in the normal control group were fed a standard diet, while the others in the model group were fed a fat-rich diet that consisted of 10% lard + 2% cholesterol + 5% corn oil + 83% standard diet. At the end of the 16th week of the experiment all rats were sacrificed and serum and hepatic tissue were collected. Concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST) were examined and the concentration of tumor necrosis factor alpha (TNF-α) in serum was measured by enzyme-linked immunosorbent assay (ELISA). Histological characters were observed by HE, Sudan IV, and Masson staining under microscopy. The expression of IKKβ mRNA and the binding activity of nuclear factor-kappa B (NF-κB) were determined by reverse transcription-polymerase chain reaction (RT-PCR) and electrophoretic mobility shift assay (EMSA), respectively ......
您现在查看是摘要介绍页，详见PDF附件(961KB，6页)。