变性梯度凝胶电泳分析检测结直肠癌APC和p53基因突变
结直肠肿瘤;APC基因;p53基因;变性梯度凝胶电泳,王艳,李岩,通讯作者:,MutationanalysisofAPCandp53genesincolorectalcancerbydenaturinggradientgelelectrop
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王艳, 中国医科大学附属盛京医院滑翔分院综合内科 辽宁省沈阳市 110021
李岩, 中国医科大学附属盛京医院消化内科 辽宁省沈阳市 110004
通讯作者: 李岩, 110004, 辽宁省沈阳市和平区三好街36号, 中国医科大学附属盛京医院消化内科. yanli0227@163.com
电话: 024-83956416
收稿日期: 2007-04-06 接受日期: 2007-05-23
Mutation analysis of APC and p53 genes in colorectal cancer by denaturing gradient gel electrophoresis
Yan Wang, Yan Li
Yan Wang, Department of Internal Medicine, Shengjing Hospital of China Medical University, Huaxiang Branch, Shenyang 110021, Liaoning Province, China
Yan Li, Department of Digestive Diseases, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
Correspondence to: Yan Li, Department of Digestive Diseases, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang 110004, Liaoning Province, China. yanli0227@163.com
Received: 2007-04-06 Accepted: 2007-05-23
Abstract
AIM: To explore the effects of APC and p53 gene mutations in colorectal carcinoma.
METHODS: PCR-DGGE and direct DNA sequencing were used to identify mutations in exon 15 of the APC gene, and exons 5 and 7 of the p53 gene in 15 cases of normal colorectal mucosa and 60 cases of sporadic colorectal tumors.
RESULTS: Abnormal electrophoretic bands were found by DGGE. Fourteen cases of APC gene mutations and 16 cases of p53 gene mutations were detected. DNA sequencing showed that 13/14 cases took place in the APC-MCR region, 9/16 at exon 5, and 7/16 at exon 7. Two cases had both APC and p53 gene mutations.
CONCLUSION: DGGE was very sensitive for screening mutations of APC and p53 genes. DGGE combined with DNA sequencing could be used to define all colorectal cancer mutations. The APC and p53 genes were both involved in the formation stages of colorectal carcinoma.
Key Words: Colorectal carcinoma; APC gene; p53 gene; Denatured gradient gel electrophoresis ......
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