SiRNA沉默livin基因表达促进胃癌细胞凋亡
RNA干扰;livin基因;SGC-7901细胞;凋亡;逆转录酶链式反应;流式细胞术,王同杉,游思洪,葛红梅,刘平,通讯作者:,InductionofapoptosisinSGC-7901cellsbysmallinterferingRNA-med
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王同杉, 游思洪, 葛红梅, 刘平, 南京医科大学第一附属医院肿瘤科 江苏省南京市 210029
王同杉, 2000年苏州大学医学院本科毕业, 2003年南京医科大学肿瘤学硕士生, 肿瘤科住院医师, 主要从事消化系肿瘤早期诊治研究.
江苏省医学重点人才135工程资助项目, No.52-2001
通讯作者: 刘平, 210029, 江苏省南京市广州路300号, 南京医科大学第一附属医院肿瘤科. liu-ping@csco.org.cn
电话: 025-83718836-6415 传真: 025-83724440
收稿日期: 2006-07-01 修回日期: 2007-07-03
Induction of apoptosis in SGC-7901 cells by small interfering RNA-mediated silencing of the livin gene
Tong-Shan Wang, Si-Hong You, Hong-Mei Ge, Ping Liu
Tong-Shan Wang, Si-Hong You, Hong-Mei Ge, Ping Liu, Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Supported by: “135 Project” for Outstanding Person in Medicine of Jiangsu Province, No.52-2001
Correspondence to: Ping Liu, Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, Jiangsu Province,China. liu-ping@csco.org.cn
Received: 2006-07-01 Revised: 2007-07-03
Abstract
AIM: To explore the feasibility of small interfering RNA (siRNA) for inhibiting livin gene expression, and to investigate the apoptotic susceptibility of SGC-7901 cells to siRNA-mediated silencing of the livin gene.
METHODS: Two siRNAs (si-livin1 and si-livin2) that specifically target the livin gene were designed based on their coding regions and were synthesized in vitro. They were then transfected into gastric cancer SGC-7901 cells. Expression level of livin mRNA was assayed by RT-PCR. Apoptosis was assessed by flow cytometry. Growth of SGC-7901 cells and 50% inhibitory concentration (IC50) of 5-fluorouracil (5-FU) and cisplatin for cell growth were analyzed by the MTT method.
RESULTS: The expression level of livin mRNA treated for 48 hours by si-livin1 was significantly decreased in SGC7901 cells (livin α, 0.167 ± 0.013 vs 0.403 ± 0.036, 0.389 ± 0 ......
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