胰岛素样生长因子Ⅱ与胃癌细胞SGC7901 c-fos和c-jun表达的关系
胃癌细胞SGC7901;胰岛素样生长因子Ⅱ;逆转录聚合酶链反应,胰岛素样生长因子Ⅱ与胃癌细胞SGC7901c-fos和c-jun表达的关系,高欣,邓长生,高欣,通讯作者:,Invitrorelationshipofc-fosandc-junexpressionwithinsulin-lik
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高欣, 邓长生, 武汉大学中南医院消化内科 湖北省武汉市 430071
高欣, 2007届武汉大学中南医院硕士研究生, 主要从事胃癌基础与临床研究.
通讯作者: 邓长生, 430071, 湖北省武汉市武昌区东湖路169号, 武汉大学中南医院消化内科. kate112@163.com收稿日期: 2007-06-09 修回日期: 2007-08-09
In vitro relationship of c-fos and c-jun expression with insulin-like growth factor-Ⅱ in SGC7901 gastric cells
Xin Gao, Chang-Sheng Deng
Xin Gao, Chang-Sheng Deng,Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China
Correspondence to:Chang-Sheng Deng, Department of Gastroenterology, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuchang District, Wuhan 430071, Hubei Province, China. kate112@163.com
Received: 2007-06-09 Revised:2007-08-09
Abstract
AIM: To study the effects of c-fos and c-jun expression in the SGC7901 gastric cancer cell line with insulin-like growth factor (IGF)-Ⅱ.
METHODS: Proliferative activity was determined by the MTT assay. c-fos and c-jun expression was detected by immunohistochemistry and reverse transcriptase-polymerase chain reaction.
RESULTS: IGF-Ⅱ promoted the proliferation of SGC7901 human gastric cancer cells. The rate of proliferation was time and dosage dependent. Increasing concentrations of IGF-Ⅱ increased c-fos and c-jun expression at the protein and mRNA level. IGF-Ⅱ at 10, 50 or 100 mg/L resulted in c-fos and c-jun protein expression of 41.32 ± 1.28, 50.43 ± 0.57 and 64.22 ± 1.76 mg/L; and 52.00 ± 0.67, 63.20 ± 0.95 and 76.31 ± 1.16 mg/L, respectively. The ratios of c-fos and c-jun expression in mRNA level with glyceraldehyde-3-phosphate dehydrogenase mRNA were 0.316 ± 0.021, 0.392 ± 0.0357 and 0.478 ± 0.028; and 0.379 ± 0.006, 0.412 ± 0.022, and 0.494 ± 0.048, respectively. The ratios of the experimental group were significantly different from those of the control group (30.00 ± 1.01 mg/L, 41.14 ± 2.02 mg/L, 0.220 ± 0.037, 0.290 ± 0.064, respectively) ......
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