研究快报 RAPID COMMUNICATION
脂多糖;谷氨酰胺;Toll样受体;逆转录-聚合酶链反应;免疫印迹,研究快报RAPIDCOMMUNICATION,荆科,孙梅,通讯作者:,EffectsofglutamineonToll-likereceptor2and4expressioninratswithintestinalendotoxem
荆科, 孙梅, 中国医科大学附属第二医院(盛京医院)儿科 辽宁省沈阳市 110004通讯作者: 孙梅, 110004, 辽宁省沈阳市和平区三好街36号, 中国医科大学附属第二医院(盛京医院)儿科. sunm@cmu2h.com
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收稿日期: 2007-05-16 修回日期: 2007-09-30
Effects of glutamine on Toll-like receptor 2 and 4 expression in rats with intestinal endotoxemia
Ke Jing, Mei Sun
Ke Jing, Mei Sun, Department of Pediatrics, the Second Affiliated Hospital (Shengjing Hospital) of China Medical University, Shenyang 110004, Liaoning Province, China
Correspondence to: Mei Sun, Department of Pediatrics, the Second Affiliated Hospital (Shengjing Hospital) of China Medical University, 36 Sanhao Street, Heping District, Shenyang 110004, Liaoning Province, China. sunm@cmu2h.com.
Received: 2007-05-16 Revised: 2007-09-30
Abstract
AIM: To determine the effect of glutamine (Gln) on Toll-like receptor (TLR) 2 and 4 expression in rats with intestinal endotoxemia.
METHODS: Twenty-four Wistar rat pups aged 10 d were randomly divided into three groups: control (normal saline 1 mL/kg ip, n = 8), lipopolysaccharide (LPS) (LPS 5 mg/kg ip, n = 8), and Gln group (LPS 5 mg/kg + Gln 10 mL/kg ip, n = 8). The rats were sacrificed at 3 h after treatment. The small intestine was dissected for histological analysis, and expression of TLR2 and TLR4 mRNA was detected by reverse transcription-polymerase chain reaction. TLR2 and TLR4 protein expression was measured by Western blotting. ......
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