镇江地区H pylori的cagA基因及其3'可变区结构的变化
幽门螺杆菌;cagA基因;CagA蛋白;EPIYA;临床结果,镇江地区Hpylori的cagA基因及其3'可变区结构的变化,吴莺,张尤历,范钰,魏金文,何亚龙,沈琰,陈劲频,印亦萍,王文兵,通讯作者:,Studyofthe3’terminalvariableregionofthecagAgene
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吴莺, 张尤历, 范钰, 魏金文, 何亚龙, 沈琰, 陈劲频, 印亦萍, 江苏大学附属医院消化科 江苏省镇江市 212001
王文兵, 江苏大学生命科学院 江苏省镇江市 212000
江苏省科学技术厅社会发展基金资助项目, No. BS2006041
通讯作者: 张尤历, 212001, 江苏省镇江市解放路438号, 江苏大学附属医院消化科. jszjwy@hotmail.com
电话: 0511-85026370
收稿日期: 2007-05-31 修回日期: 2007-09-18
Study of the 3’ terminal variable region of the cagA gene in Helicobacter pylori isolated from patients living in Zhenjiang
Ying Wu, You-Li Zhang, Yu Fan, Jin-Wen Wei, Ya-Long He, Yan Shen, Jin-Pin Chen, Yi-Ping Yin, Wen-Bing Wang
Ying Wu, You-Li Zhang, Yu Fan, Jin-Wen Wei, Ya-Long He, Yan Shen, Jin-Pin Chen, Yi-Ping Yin, Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Wen-Bing Wang, Institute of Life Science, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Supported by: Social Development Program of Department of Science and Technology, Jiangsu Province, No. BS2006041
Correspondence to: You-Li Zhang, Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, Jiangsu Province, China. zjyouli@yahoo.com.cn
Received: 2007-05-31 Revised: 2007-09-18
Abstract
AIM: To evaluate the expression of cagA gene of Helicobacter pylori strains isolated from Chinese patients living in Zhenjiang, Jiangsu Province, their CagA protein type, and the relationship between the number of EPIYA located in CagA protein C terminus and the clinical outcome.
METHODS: After H pylori isolated from patients with gastrointestinal diseases were cultured, the genomic DNA was extracted and PCR was performed to examine the expression of the variable region of the cagA gene. The DNA fragments from 20 isolated strains with cagA+ were sequenced.
RESULTS: CagA gene expression was found in 56 of 60 patients. According to the amino acid sequences of CagA protein variable region, the isolates fell into two groups: the East-Asia type that contained 19 isolated strains belonging to Yamaoka’s A type, and one strain that belonged to the Western type ......
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