曲格列酮诱导胃癌MKN45细胞株凋亡
曲格列酮;过氧化物酶体增殖激活受体γ;胃癌;凋亡;EMSA;流式细胞术,蒋春舫,陈庆,郑海,周洁,陈娟,蒋春舫,通讯作者:,TroglitazoneinducesgastriccancercelllineMKN45apoptosis,Chun-Fang
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蒋春舫, 陈庆, 郑海, 华中科技大学附属协和医院普外科 湖北省武汉市 430022
周洁, 陈娟, 华中科技大学同济医学院生物化学与分子生物学系 湖北省武汉市 430030
蒋春舫, 硕士, 副教授, 主要从事胃肠疾病的治疗和研究工作.
湖北省卫生厅科研基金资助项目, No. 2003JX1B006
通讯作者: 蒋春舫, 430022, 湖北省武汉市解放大道1277号, 华中科技大学附属协和医院普外科. jiangcfun@126.com
电话: 027-85351619
收稿日期: 2007-03-04 修回日期: 2007-10-04
Troglitazone induces gastric cancer cell line MKN45 apoptosis
Chun-Fang Jiang, Qing Chen, Jie Zhou, Hai Zheng, Juan Chen
Chun-Fang Jiang, Qing Chen, Hai Zheng, Department of General Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Jie Zhou, Juan Chen, Department of Biochemistry & Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Supported by: the Scientific Research Fund from the Health Department of Hubei Province, No. 2003JX1B006
Correspondence to: Chun-Fang Jiang, Department of General Surgery, Union Hospital, Huazhong University of Science and Technology, 1277 Jiefang Road, Wuhan 430022, Hubei Province, China. jiangcfun@126.com
Received: 2007-03-04 Revised: 2007-10-04
Abstract
AIM: To observe the effects of different concentrations of troglitazone on the activity of peroxisome proliferators-activated receptor γ (PPARγ), and apoptosis of the MKN45 gastric cell line.
METHODS: Cultured MKN45 gastric cells were randomly divided into four groups: control, 1, 5 and 10 μmol/L troglitazone. The change in activity of PPARγ was detected by electrophoretic mobility shift assay (EMSA). Flow cytometry was used to detect changes in the expression of caspase-3, cell cycle and apoptosis.
RESULTS: TPPARγ activity of cultured MKN45 gastric cells increased significantly with the concentration of troglitazone. Data were quantified for the control group as 100, the mean activity of 1 μmol/L troglitazone was 155 ......
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