胃癌组织中caspase 9的表达及其多态性变化
胃癌;Caspase9;单核苷酸多态;限制性片段长度多态性技术,胃癌组织中caspase9的表达及其多态性变化,方长清,刘树立,李建华,娄毅,方长清,通讯作者:,Expressionofthecaspase9geneanditspolymorphismdistributioningastr
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方长清, 刘树立, 李建华, 中国医科大学病理教研室 辽宁省沈阳市 110001
娄毅, 中国医科大学医学遗传学教研室 辽宁省沈阳市 110001
方长清, 主管技师, 主要从事肿瘤的研究工作.
辽宁省科技基金资助, No. 2005225003-6
通讯作者: 李建华, 110001, 辽宁省沈阳市, 中国医科大学病理教研室. louyi_1963_1019@163.com
电话: 024-23256666
收稿日期: 2007-07-13 修回日期: 2007-09-27
Expression of the caspase 9 gene and its polymorphism distribution in gastric cancer
Chang-Qing Fang, Shu-Li Liu, Yi Lou, Jian-Hua Li
Chang-Qing Fang, Shu-Li Liu, Jian-Hua Li,Department of Pathology, China Medical University, Shenyang 110001, Liaoning Province, China
Yi Lou, Department of Medical Genetics, China Medical University, Shenyang 110001, Liaoning Province, China
Supported by: Science and Technology Fund of Liaoning Province, No. 2005225003-6
Correspondence to: Jian-Hua Li, Department of Pathology, China Medical University, Shenyang 110001, Liaoning Province, China. louyi_1963_1019@163.com
Received: 2007-07-13 Revised: 2007-09-27
Abstract
AIM: To explore the expression of caspase 9 in gastric cancer, and single nucleotide polymorphism (SNP) distribution of caspase 9 in gastric cancer patients and healthy individuals.
METHODS: Western blotting was used to analyze the expression of caspase 9 in 70 gastric cancer patients and normal gastric tissues. We investigated the distribution of a caspase 9 gene SNP. We analyzed the genotype rs1052576 in 70 patients and 100 healthy individuals by polymerase chain reaction-restriction fragment length polymorphism. Statistical analysis was applied to analyze SNP genotype frequency and allele frequency in patients and the control group.
RESULTS: In comparison with normal gastric tissues, caspase 9 gene expression was obviously down-regulated in 51.4% (36/70) of gastric cancer tissues. rs1052576 was located in exon 5 of the caspase 9 gene showed a significant difference between the two groups, and the G allele frequency in gastric cancer patients was higher than that in healthy controls (22 ......
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